Ontology highlight
ABSTRACT:
PROVIDER: PRJEB24491 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Ice nucleating bacteria from Arctic cloud water
| PRJEB30621 | ENA
Project description:<p>Antimicrobial resistance (AMR) is one of the most urgent challenges in public health, and the horizontal transfer of antibiotic resistance genes (ARGs) mediated by mobile genetic elements (MGEs) drives its widespread global dissemination. Although integrative conjugative elements (ICEs) outnumber conjugative plasmids, research on ICE-mediated horizontal gene transfer (HGT) remains largely lacking, especially concerning SXT ICEs, which are highly prevalent, pose significant risks, and are directly related to human health. Cyromazine is a widely used feed additive, with high detection rates in the feces of farmed animals and related environments. It has also been shown to increase the abundance of ARGs, and the potential driving mechanisms urgently require further exploration. Thus, we investigated the impact of cyromazine on SXT ICE-mediated ARGs transfer and elucidated its underlying mechanisms. Our study demonstrates that cyromazine promotes SXT ICE conjugative transfer both intra- and inter-species. This facilitating effect confirmed in mouse intraperitoneal and intestinal conjugation models. The potential mechanisms include cyromazine-induced ROS accumulation and triggering of the SOS response, which could promote SXT ICE excision and activate conjugation-related operons. Furthermore, enhanced energy metabolism and altered lipid metabolism further facilitate SXT ICE transfer. Collectively, our study partially fills the gap in understanding ICE-mediated AMR transmission dynamics, and highlighting that the exacerbated AMR crisis is associated with cyromazine-stimulated high-frequency SXT ICE conjugation in vitro and in vivo.</p>
2025-10-15 | MTBLS13146 | MetaboLights
Project description:Whole Genome Sequencing and Hybrid Assembly of an ice-nucleating air isolate of Pantoea ananatis
| PRJEB121357 | ENA
Project description:Linking Biogenic High-Temperature Ice Nucleating Particles in Arctic soils and Streams to Their Microbial Producers
| PRJNA1137255 | ENA
Project description:Airborne ultrafine particles (UFP) constitute a potential risk factor for human health, being transport one of the most relevant sources, particularly in urban environments. We collected UFP from outdoor sites in Barcelona and Valencia (Spain) selected to distinguish between the contributions of road traffic, trains, ships, and planes. Zebrafish embryos were exposed to organic extracts from the collection filters, and their transcriptomes were analysed by RNA deep sequencing. Functional analysis of 2,941 significantly affected transcripts revealed a strong enrichment in functions related to general embryotoxicity, like induction of degradation pathways (endocytosis, necroptosis), and inhibition of development- and cell division-related genes. This embryotoxicity-related transcriptional response was linked to elevated concentrations of UFP, sulfur, and other inorganic species in the air samples, and it was particularly strong in UFP-rich airport samples. In addition, the functional analysis also identified a relatively small subset of genes configuring a typical dioxin-like response, traditionally associated with exposure to airborne combustion particles, and that was linked to PAH-rich emissions from harbour and traffic samples. Consistently, relative abundance of the implicated genes showed a strong correlation with high concentrations of polycyclic aromatic hydrocarbons (PAHs) in the extracts. Our findings indicate that the parameters currently used to assess air quality overlook the role of UFP in total airborne toxicity and, consequently, they do not adequately protect human populations.
2026-04-14 | GSE320027 | GEO
Project description:Acutely activated enhancers require the actions of Topo1cc, including those induced by 17β-oestradiol (E2), dihydrotestosterone (DHT) and tumor necrosis factor alpha (TNFa). Topo1cc at these acutely activated enhancers is “read” by Ku70, which in this context serves as a required transcriptional coactivator, based on nucleating a Ku70/heterochromatin protein1 gamma (HP1γ) complex to facilitate the recruitment of mediator subunit Med26 for eRNA transcription.
2022-08-04 | GSE190284 | GEO