Characterization of microbial communities during a Ground Tire Rubber (GTR) desulfurization process
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB24536 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR2262080_1.fastq.gz | Fastqsanger.gz | |||
| ERR2262080_2.fastq.gz | Fastqsanger.gz | |||
| ERR2262081_1.fastq.gz | Fastqsanger.gz | |||
| ERR2262081_2.fastq.gz | Fastqsanger.gz | |||
| ERR2262082_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 14 |
Similar Datasets
Project description:Desulfurization wastewater treatment process Raw sequence reads
| PRJNA687878 | ENA
Project description:We devulcanized ground tire rubber (GTR) in a laboratory microwave oven and an internal mixer, measured the soluble content and the cross-link density of the samples, and then used Horikx’s analysis. The results showed that microwave treatment caused severe degradation of the polymer chains, while in the case of thermomechanical devulcanization, the selective scission of covalent cross-links is more common. Four devulcanized ground tire rubber (dGTR) samples were chosen for further study and three groups of samples were produced: dGTR samples containing vulcanizing agents and different amounts of paraffin oil (dGTR-based mixtures), natural rubber-based rubber mixtures with different dGTR contents and reference rubber mixtures with dGTR-based mixtures (increased vulcanizing agent contents). Cure characteristics showed a plasticizer-like effect of dGTR. Tensile and tear strength decreased drastically with increasing dGTR content; however, elongation at break values did not follow such a trend. Mechanical properties improved with increased vulcanizing agent contents. The examined properties of the samples improved even further with the use of thermomechanically devulcanized samples. Horikx’s analysis showed that this is attributable to moderate polymer chain scission.
| S-EPMC7538573 | biostudies-literature
Project description:In this paper, low-temperature extrusion of ground tire rubber was performed as a pro-ecological waste tires recycling method. During this process, ground tire rubber was modified with constant content of dicumyl peroxide and a variable amount of elastomer (in the range: 2.5-15 phr). During the studies, three types of elastomers were used: styrene-butadiene rubber, styrene-ethylene/butylene-styrene grafted with maleic anhydride and ethylene-octene copolymer. Energy consumption measurements, curing characteristics, physico-mechanical properties and volatile organic compounds emitted from modified reclaimed GTR were determined. The VOCs emission profile was investigated using a passive sampling technique, miniature emission chambers system and static headspace analysis and subsequently quantitative or qualitative analysis by gas chromatography. The VOCs analysis showed that in the studied conditions the most emitted volatile compounds are dicumyl peroxide decomposition by-products, such as: α-methylstyrene, acetophenone, α-cumyl alcohol, methyl cumyl ether, while the detection level of benzothiazole (devulcanization "marker") was very low. Moreover, it was found that the mechanical properties of the obtained materials significantly improved with a higher content of styrene-butadiene rubber and styrene-ethylene/butylene-styrene grafted with maleic anhydride while the opposite trend was observed for ethylene-octene copolymer content.
| S-EPMC8839703 | biostudies-literature
Project description:As a means to decrease the amount of waste tires and to re-use tire rubber for new tires, devulcanization of ground passenger car tires is a promising process. Being an established process for NR and EPDM, earlier work has shown that for ground passenger car tire rubber with a relatively high amount of SBR, a devulcanization process can be formulated, as well. This was proven for a laboratory-scale batch process in an internal mixer, using diphenyl disulfide as the devulcanization aid and powder-sized material. In this paper, the devulcanization process for passenger car tire rubber is upscaled from 15 g per batch and transformed into a continuous process in a co-rotating twin screw extruder with a capacity of 2 kg/h. As SBR is rather sensitive to devulcanization process conditions, such as thermal and mechanical energy input, the screw design was based on a low shear concept. A granulate with particle sizes from 1-3.5 mm was chosen for purity, as well as economic reasons. The devulcanization process conditions were fine-tuned in terms of: devulcanization conditions (time/temperature profile, concentration of devulcanization aid), extruder parameters (screw configuration, screw speed, fill factor) and ancillary equipment (pre-treatment, extrudate handling). The influence of these parameters on the devulcanization efficiency and the quality of the final product will be discussed. The ratio of random to crosslink scission as determined by a Horikx plot was taken for the evaluation of the process and material. A best practice for continuous devulcanization will be given.
| S-EPMC5457053 | biostudies-other
Project description:TIRE-seq (Turbocapture Integrated RNA Expression Sequencing) is a novel RNA sequencing method that integrates mRNA purification directly into library preparation, eliminating separate RNA extraction. Compared to traditional protocols like Primeseq, TIRE-seq demonstrates improved sequencing efficiency using crude cell lysates. We validated the method across three biological applications: analyzing transcriptional changes in stimulated human T cells, identifying genes driving murine dendritic cell differentiation, and examining temozolomide's dose-response effects on patient-derived neurospheres. TIRE-seq offers a streamlined, cost-effective approach for gene expression studies, reducing workflow complexity and minimizing sample loss.
2024-12-19 | GSE284395 | GEO
Project description:Inadequate fire resistance of polymers raises questions about their advanced applications. Flexible polyurethane (PU) foams have myriad applications but inherently suffer from very high flammability. Because of the dependency of the ultimate properties (mechanical and damping performance) of PU foams on their cellular structure, reinforcement of PU with additives brings about further concerns. Though they are highly flammable and known for their environmental consequences, rubber wastes are desired from a circularity standpoint, which can also improve the mechanical properties of PU foams. In this work, melamine cyanurate (MC), melamine polyphosphate (MPP), and ammonium polyphosphate (APP) are used as well-known flame retardants (FRs) to develop highly fire-retardant ground tire rubber (GTR) particles for flexible PU foams. Analysis of the burning behavior of the resulting PU/GTR composites revealed that the armed GTR particles endowed PU with reduced flammability expressed by over 30% increase in limiting oxygen index, 50% drop in peak heat release rate, as well as reduced smoke generation. The Flame Retardancy Index (FRI) was used to classify and label PU/GTR composites such that the amount of GTR was found to be more important than that of FR type. The wide range of FRI (0.94-7.56), taking Poor to Good performance labels, was indicative of the sensitivity of flame retardancy to the hybridization of FR with GTR components, a feature of practicality. The results are promising for fire protection requirements in buildings; however, the flammability reduction was achieved at the expense of mechanical and thermal insulation performance.
| S-EPMC10934310 | biostudies-literature
Project description:The application of wastes as a filler/reinforcement phase in polymers is a new strategy to modify the performance properties and reduce the price of biocomposites. The use of these fillers, coming from agricultural waste (cellulose/lignocellulose-based fillers) and waste rubbers, constitutes a method for the management of post-consumer waste. In this paper, highly-filled biocomposites based on natural rubber (NR) and ground tire rubber (GTR)/brewers' spent grain (BSG) hybrid reinforcements, were prepared using two different curing systems: (i) sulfur-based and (ii) dicumyl peroxide (DCP). The influence of the amount of fillers (in 100/0, 50/50, and 0/100 ratios in parts per hundred of rubber) and type of curing system on the final properties of biocomposites was evaluated by the oscillating disc rheometer, Fourier-transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, swelling behavior, tensile testing, and impedance tube measurements. The results show, that the scorch time and the optimum curing time values of sulfur cured biocomposites are affected by the change of the hybrid filler ratio while using the DCP curing system, and the obtained values do not show significant variations. The results conclude that the biocomposites cured with sulfur have better physico-mechanical and acoustic absorption, and that the type of curing system does not influence their thermal stability. The overall analysis indicates that the difference in final properties of highly filled biocomposites cured with two different systems is mainly affected by the: (i) cross-linking efficiency, (ii) partial absorption and reactions between fillers and used additives, and (iii) affinity of additives to applied fillers.
| S-EPMC7182860 | biostudies-literature
Project description:The use of devulcanized tire powder as an effective reinforcement in self-healing styrene-butadiene rubber (SBR) compounds has been investigated for the first time in this work. For this purpose, the evolution of the microstructure of the rubber from end-of-life tires (ELTs) was studied during granulation, grinding and devulcanization through an exhaustive characterization work in order to relate the final microstructure with the mechanical response of the repaired systems. Different morphologies (particle size distribution and specific surface area) obtained by cryogenic and water jet grinding processes, as well as different devulcanization techniques (thermo-mechanical, microwave, and thermo-chemical), were analyzed. The results demonstrated the key influence of the morphology of the ground tire rubber (GTR) on the obtained devulcanized products (dGTR). The predictions of the Horikx curves regarding the selectivity of the applied devulcanization processes were validated, thereby; a model of the microstructure of these materials was defined. This model made it possible to relate the morphology of GTR and dGTR with their activity as reinforcement in self-healing formulations. In this sense, higher specific surface area and percentage of free surface polymeric chains resulted in better mechanical performance and more effective healing. Such a strategy enabled an overall healing efficiency of more than 80% in terms of a real mechanical recovery (tensile strength and elongation at break), when adding 30 phr of dGTR. These results open a great opportunity to find the desired balance between the mechanical properties before and after self-repair, thus providing a high technological valorization to waste tires.
| S-EPMC8747371 | biostudies-literature
Project description:The circular leaf disease, caused by Pestalotiopsis spp., is a new primary foliar disease characterized by yellow-brown spots that develop into dark brown spots on rubber leaves. This disease, called leaf fall disease, has recently caused significant economic losses in countries that produce natural rubber. However, the molecular mechanisms involved in the interaction between P. microspora and H. brasiliensis are not well understood. In this study, we carried out transcriptome analysis by RNA-Seq of a susceptible rubber clone (GT-1) after inoculation with the mycelial plug of P. microspora. The analysis brought us to identify 3,799, 7,274, and 4,678 differentially expressed genes (DEGs) at 2, 4, and 6 days post infection (dpi), respectively. In addition to general hypersensitive response (HR) and systemic acquired response (SAR) mediated by salicylic acid and hydrogen peroxide, our Gene Ontology (GO) analysis of the detected DEGs revealed abscisic acid and ethylene responses. Detection of these two phytohormone responses suggests possible involvement of abscisic acid and/or ethylene in the process to lead the leaf fall in susceptible rubbers. Activation of the ethylene signaling by the pathogen infection was also supported by our promoter analysis, which detected GCC box in the putative cis-elements for the response to P. microspora infection. Regarding the down-regulated genes, GO analyses showed suppression of photosynthesis across the infection time points. In addition to global understanding of the transcriptional response of rubber to the pathogen, we extracted general Regulatory Element Group (REG) that are position-dependent cis-regulatory elements and covers half of the elements in a genome, and also established molecular markers for qRT-PCR. Our study provides genomic information and convenient tools for facilitating further exploration of Hevea-Pestalotiopsis interaction
2026-01-05 | GSE310758 | GEO