Project description:Microalgae and cyanobacteria represent an emerging and sustainable source of bioactive compounds for the food, cosmeceutical, and pharmaceutical sectors. In this study, the potential of two microalgae-cyanobacteria consortia, consortium 1 (C1) consisting of Chlorella vulgaris and Arthrospira platensis, and consortium 2 (C2) consisting of Kamptonema sp., Nannochloropsis oculata, Tetraselmis suecica, and Chlorella vulgaris, as a source of bioactive peptides was evaluated. Firstly, protein extraction from both biomasses was optimized by testing different protein solubilization and precipitation pHs, with pH 10 and pH 5 providing the best results in terms of protein recovery in both cases. Selected protein extracts, with protein contents of 28.50 ± 2.69 % (C1) and 8.46 ± 0.45 % (C2), were further hydrolyzed with Alcalase, evaluating the impact of the incubation time on peptide release and the antioxidant capacity of hydrolysates. 1 h of hydrolysis proved to be enough for antioxidant capacity increase. In addition, in silico hydrolysis with Alcalase of the proteins identified in C1 and C2 was evaluated, assessing the potential bioactivity of the peptides produced, more specifically their antioxidant capacity. Our findings demonstrate that both microalgae-cyanobacteria consortia are valuable sources of bioactive compounds with antioxidant capacity, with potential interest as functional ingredients for the food, cosmeceutical, and pharmaceutical industries.
Project description:Microalgae and cyanobacteria represent an emerging and sustainable source of bioactive compounds for the food, cosmeceutical, and pharmaceutical sectors. In this study, the potential of two microalgae-cyanobacteria consortia, consortium 1 (C1) consisting of Chlorel-la vulgaris and Arthrospira platensis, and consortium 2 (C2) consisting of Kamptonema sp., Nannochloropsis oculata, Tetraselmis suecica, and Chlorella vulgaris, as a source of bioactive peptides was evaluated. Firstly, protein extraction from both biomasses was optimized by testing different protein solubilization and precipitation pHs, with pH 10 and pH 5 providing the best results in terms of protein recovery in both cases. Selected protein ex-tracts, with protein contents of 28.50 ± 2.69 % (C1) and 8.46 ± 0.45 % (C2), were further hy-drolyzed with Alcalase, evaluating the impact of the incubation time on peptide release and the antioxidant capacity of hydrolysates. 1 h of hydrolysis proved to be enough for antioxidant capacity increase. In addition, in silico hydrolysis with Alcalase of the proteins identified in C1 and C2 was evaluated, assessing the potential bioactivity of the peptides produced, more specifically their antioxidant capacity. Our findings demonstrate that both microalgae-cyanobacteria consortia are valuable sources of bioactive compounds with antioxidant capacity, with potential interest as functional ingredients for the food, cosmeceutical, and pharmaceutical industries.
Project description:Arthrospira platensis C1, a filamentous cyanobacterium, is one of the most commercially successful microalgae. However, high temperature stress is a critical problem that alters metabolic functions and causes irreversible damage during the growth of microorganisms, resulting in a loss of biomass productivity and high-value compounds. The investigation of genome-wide expression changes can provide towards a better understanding on the cellular stress responses.
Project description:Identification of functional genes involved in various molecular, biological and cellular process from the transcriptome dataset of Arthrospira platensis
