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RNAseq of the spleen of Hbb(th3/+) mice transplanted with bone marrow from Tfr2-ko or Tfr2 wt mice

ABSTRACT: RNAseq of the spleen of Hbb(th3/+) mice transplanted with bone marrow from Tfr2-ko or Tfr2 wt mice

PROVIDER: PRJEB25653 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2433074_1.fastq.gz	Fastqsanger.gz
	ERR2433074_2.fastq.gz	Fastqsanger.gz
	ERR2433075_1.fastq.gz	Fastqsanger.gz
	ERR2433075_2.fastq.gz	Fastqsanger.gz
	ERR2433076_1.fastq.gz	Fastqsanger.gz

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RNAseq of the spleen of Hbb(th3/+) mice transplanted with bone marrow from Tfr2-ko or Tfr2 wt mice

Project description:C57Bl/6 Ly5.1 male mice lethally irradiated have been transplanted at 8 weeks of age with bone marrow from Hbb(th3/+)/Tfr2(+/+) or Hbb(th3/+)/Tfr2(-/-) mice (Bone marrow in a mixed genetic background C57BL6/129Sv). After transplantation, the mice have been maintained in a standard diet for 22 weeks and euthanised for organs extraction and analysis.

2020-03-04 | E-MTAB-6606 | biostudies-arrayexpress

Label-free quantitative proteomics analysis of bone marrow cells of MK-LCM WT versus KO mice

Project description:High ploidy large cytoplasmic megakaryocytes (LCM) are critical negative regulators of hematopoietic stem cells (HSC) and are responsible for platelet formation. Using a mouse knockout model with normal megakaryocyte numbers but essentially devoid of LCM (MK-LCM KO), we demonstrated a pronounced increase in bone marrow HSC concurrent with endogenous mobilization and extramedullary hematopoiesis. When HSC isolated from a MK-LCM KO microenvironment were transplanted in lethally irradiated mice, the absence of LCM increased HSC in BM, blood and spleen. Severe thrombocytopenia was observed in animals with diminished LCM, although there was no change in megakaryocyte ploidy distribution. In contrast, WT HSC-generated LCM regulated a normal HSC pool and prevented thrombocytopenia. The present label-free quantitative LC-MSMS data was used to determine proteins that are differentially expressed in bone marrow cells of MK-LCM WT versus MK-LCM KO mice.

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Ancestral beta-globin gene haplotypes modify beta-thalassemia severity in a mouse model [Ery]

Project description:beta-Thalassemia is a prevalent anemia caused by mutations in the HBB (beta-globin) gene. We show that the severity of beta-thalassemia in the frequently studied Hbb(th3/+) mouse model is influenced by ancestral beta-globin gene (Hbb) haplotypes that differ in common strains.

2024-12-18 | GSE260900 | GEO

The Effect of liver regeneration on hESCs transplanted into SCID mice

Project description:A source of functioning hepatocytes for liver cell transplantation and liver support is in need. hESCs , when transplanted, generally form teratomas. We studied capacity of hESCs to differentiate to hepatocyte like cells under the effect of in vivo liver regeneration. In SCID-Beige mice hepatocyte replication peaked 48 hours after CCl4 injection; 24 hours earlier 106 hESCs or EBs at different stages of differentiation were transplanted into the spleen. Comparisons were made to teratomas formed in the hind limb of untreated animals. RT-PCR and gene microarray were used for liver and human specific markers. Immunohistochemistry to AFP,AAT, ALB, HEP-PAR I AND CK-18,19 were performed. EBs formed a single large teratoma in the spleen and small teratomas in the liver. Expression of PCR- identified liver specific markers was greater in the spleen than in the liver. Adult hepatocyte specific markers were expressed in the hind limb teratoma excised after 7 weeks. When late EBs were transplanted before CCl4 exposure, no teratomas formed. Rather, an abundance of probably undifferentiated ectodermal origin cells presented. In this descriptive study, transplanted early human EBs formed teratomas that differed in size and molecular markers. Within teratomas, the degree of maturation into hepatocytes correlated better with the time duration in vivo than with growth stimulation. Late EBs formed non differentiated ectodermal cells only in a regenerative microenvironment. 4 samples were analyzed. Clean mouse liver used as neg. control. Mouse liver injected with CCl4 and transplanted with late Ebs, tumor was not observed. Two mouse livers injected with CCl4 and transplanted with late Ebs, tumor was observed.

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E-MTAB-6606

Project description:RNAseq of the spleen of Hbb(th3/+) mice transplanted with bone marrow from Tfr2-ko or Tfr2 wt mice

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Mice Spleen macrophages heterogeneity

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The Effect of liver regeneration on hESCs transplanted into SCID mice

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Proteomics of prostate cancer orthotopic transplanted mice

Project description:We profiled the proteomics of prostate cancer orthotopic transplanted mice.

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