Project description:We reported the microbial communities in wastewater between conventional membrane bioreactor (MBR) system and biofilm MBR system using Illumina sequencing.
Project description:Membrane bioreactor (MBR) systems are typically known different from conventional activated sludge (CAS) systems in operational parameters, while current knowledge of their microbial differentiations is barely sufficient. To this end, the current study was launched to address the differences of the overall functional genes of an oxidation ditch (OD) and an MBR running parallelly at full-scale using a functional gene array-GeoChip 4.2.
2016-03-01 | GSE67307 | GEO
Project description:Activated sludge from A2O-MBR process
Project description:Membrane bioreactor (MBR) systems are typically known different from conventional activated sludge (CAS) systems in operational parameters, while current knowledge of their microbial differentiations is barely sufficient. To this end, the current study was launched to address the differences of the overall functional genes of an oxidation ditch (OD) and an MBR running parallelly at full-scale using a functional gene array-GeoChip 4.2. Two full-scale wastewater treatment systems applying the processes of oxidation ditch (OD) and membrane bioreactor (MBR) were investigated. They treated identical wastewater at the same scale. 12 mixed-liquor suspended sludge (MLSS) samples collected daily on 12 consecutive days from each system were analyzed by GeoChip 4.2.
Project description:In this study, we integrated metagenome sequencing and metaproteomics to obtain comprehensive taxonomic and functional profiles of microbial communities, investigating the impact of stagnation compared with continuous flow dynamics. To achieve this, we employed laboratory-scale reactors fed with membrane bioreactor (MBR) effluent. The objectives were to evaluate: (i) how stagnation affects the diversity and taxonomic composition of microbial genes and proteins; (ii) how prolonged stagnation over three, five, and seven months drives temporal changes in community and functional dynamics; (iii) which functions are enriched or depleted under stagnation; and (iv) how these taxonomic and functional shifts relate to the ecological quality and biostability of reclaimed water. By understanding not only what microbes are present but also what they are doing through metaproteomics, we can assess how microbial activities impact water quality. This approach enables evaluation of treated wastewater quality both post-treatment and during distribution and storage in irrigation systems.
