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Effect of MHC II expression in pro-B cells: F6 leukaemic cell line

ABSTRACT: Effect of MHC II expression in pro-B cells: F6 leukaemic cell line

PROVIDER: PRJEB26756 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2581092_1.fastq.gz	Fastqsanger.gz
	ERR2581092_2.fastq.gz	Fastqsanger.gz
	ERR2581093_1.fastq.gz	Fastqsanger.gz
	ERR2581093_2.fastq.gz	Fastqsanger.gz
	ERR2581094_1.fastq.gz	Fastqsanger.gz

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Effect of MHC II expression in pro-B cells: F6 leukaemic cell line

Project description:F6 murine leukaemic cells were transfected either with MHC II or with GFP and transcriptionally profiled by RNA-seq. Triplicate samples for each genotype were sequenced. This is part of ongoing investigation of a possible cell-intrinsic role of MHC II B cells.

2018-08-07 | E-MTAB-6763 | biostudies-arrayexpress

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Project description:Effect of MHC II expression in pro-B cells: F6 leukaemic cell line

2018-09-17 | E-MTAB-6763 | ExpressionAtlas

Effect of MHC II expression in pro-B cells: Pre-B cells and follicular B cells from wild-type and MHC II conditional mice

Project description:Hardy’s Fraction D bone marrow pre-B cells and splenic follicular B cells were purified by flow cytometry according to MHC II expression from wild-type and ItgaxcreH2-Ab1c MHC II conditional mice and transcriptionally profiled by RNA-seq. Duplicate or triplicate samples for each genotype and level of MHC II expression were sequenced. This is part of ongoing investigation of a possible cell-intrinsic role of MHC II B cells.

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Project description:F1 hybrids can outperform their parents in yield and vegetative biomass, features of hybrid vigor which form the basis of the hybrid seed industry. The yield advantage of the F1 is lost in the F2 and subsequent generations. In Arabidopsis, from F2 plants which have a F1 –like phenotype, we have by recurrent selection produced pure breeding F5/F6 lines “Hybrid Mimics”, in which the characteristics of the F1 Hybrid are stabilized. These Hybrid Mimic lines, like the F1 Hybrid, have larger leaves than the parent plant, the leaves having increased photosynthetic cell numbers, and in some lines increased size of cells, suggesting an increased supply of photosynthate. A comparison of the differentially expressed genes in the F1 Hybrid with those of eight Hybrid Mimic lines has identified metabolic pathways altered in both; these pathways include down regulation of defense response pathways and altered abiotic response pathways. F6 Hybrid Mimic lines are mostly homozygous at each locus in the genome yet retain the large F1-like phenotype. Many alleles in the F6 plants, when they are homozygous, have expression levels different to the level in the parent. We consider this altered expression to be a consequence of trans-regulation of genes from one parent by genes from the other parent. Transregulation could also arise from epigenetic modifications in the F1. The pure breeding Hybrid Mimics have been valuable in probing the mechanisms of hybrid vigor and may also prove to be useful hybrid vigor equivalents in agriculture.

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Effect of MHC II expression in pro-B cells: Pre-B cells and follicular B cells from wild-type and MHC II conditional mice

Project description:Effect of MHC II expression in pro-B cells: Pre-B cells and follicular B cells from wild-type and MHC II conditional mice

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