Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Paramecium mating type mutants

ABSTRACT: DNA sequencing of Paramecium tetraurelia mating type mutants

PROVIDER: PRJEB27002 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2600616.fastq.gz	Fastqsanger.gz
	ERR2600617.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Transcriptome analysis, Paramecium tetraurelia ICL (as a control) vs. Rdr3 silencing

Project description:Differential transcriptome of Paramecium tetraurelia strain 51 undergoing RNAi by feeding against ICL7a (as a control) and RDR3 for nine days.

2014-07-15 | GSE59390 | GEO

DNA 5-methylcytosine in Paramecium

Project description:5-methyl-cytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization via nuclear dimorphism, are largely uncharted. While canonical cytosine methyltransferases have not been discovered in published ciliate genomes, recent studies performed in the stichotrichous ciliate Oxytricha trifallax suggest de novo cytosine methylation during macronuclear development. In this study, we applied bisfulfite genome sequencing, DNA mass spectrometry and antibody-based fluorescence detection to investigate the presence of DNA methylation in Paramecium tetraurelia. While the antibody-based methods suggest cytosine methylation, DNA mass spectrometry and bisulfite sequencing reveal that levels are actually below the limit of detection. Our results suggest that Paramecium does not utilize 5-methyl-cytosine DNA methylation as an integral part of its epigenetic arsenal.

2018-04-01 | GSE111621 | GEO

Mass spectrometry of PtGtsf1 interacted proteins in Paramecium tetraurelia

Project description:PtGtsf1 was fused with FlagHA and induced to express in Paramecium tetraurelia. The interacted proteins of PtGtsf1 was obtained by immunoprecipitation with anti-HA antibody, followed with mass spectrometry to identify the proteins. Meanwhile, FlagHA without PtGtsf1 was expressed in Paramecium tetraurelia as control and was performed with same procedures as PtGtsf1-FlagHA. By comparing the enrichment of proteins in PtGtsf1-FlagHA and control, the interacted proteins of PtGtsf1 in Paramecium tetraurelia were identified.

2025-05-06 | PXD049029 | Pride

Paramecium tetraurelia

Project description:Paramecium tetraurelia reciliation (stationary phase)

| PRJNA154019 | ENA

Paramecium tetraurelia

Project description:Paramecium tetraurelia trichocyst exocytosis recovery

| PRJNA153887 | ENA

Paramecium tetraurelia

Project description:Paramecium tetraurelia autogamy series 4

| PRJNA153891 | ENA

Paramecium tetraurelia

Project description:Paramecium tetraurelia autogamy series 3