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RNA-seq of Candida albicans Hda1 complex mutants (hda1∆∆, hda2∆∆ and hda3∆∆) and wildtype SN152 in yeast and hyphae inducing conditions

ABSTRACT: RNA-seq of Candida albicans Hda1 complex mutants (hda1∆∆, hda2∆∆ and hda3∆∆) and wildtype SN152 in yeast and hyphae inducing conditions

PROVIDER: PRJEB27358 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2652140.fastq.gz	Fastqsanger.gz
	ERR2652131.fastq.gz	Fastqsanger.gz
	ERR2652141.fastq.gz	Fastqsanger.gz
	ERR2652132.fastq.gz	Fastqsanger.gz
	ERR2652142.fastq.gz	Fastqsanger.gz

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RNA-seq of Candida albicans Hda1 complex mutants (hda1∆∆, hda2∆∆ and hda3∆∆) and wildtype SN152 in yeast and hyphae inducing conditions

Project description:Candida albicans is a normally commensal yeast which becomes pathogenic to human hosts under some conditions and specifically in immune compromised individuals. This contributes to a global health crisis of fungal disease. This yeast can morphologically adapt to the environment by reversibly developing hyphae which allows for traversing of tissue. Histone deacetylase 1 (Hda1) is involved in this transition as it is established that the deletion of the associated gene stunts this morphological switch. In Saccharomyces cerevisiae this protein complexes with two similarly named proteins: Hda2 and Hda3. Here we evaluate Candida albicans strains with mutations in the genes that code for these proteins. The strains are grown in yeast (YPD 30 °C) and hyphae (RPMI 37 °C) -inducing conditions for 90 minutes prior to RNA extraction. After alignment by HISAT2, the homozygous mutants are then individually compared to wildtype (matching growth conditions) using DESeq2.

2023-03-01 | E-MTAB-6920 | biostudies-arrayexpress

Piezo1 initiates host immune defense against pathogenic fungi through mechanosensory recognition of Candida albicans hyphae

Project description:Candida albicans metamorphoses from benign yeast to a rigid hyphae , becoming an opportunistic pathogen in immunocompromised patients. The process by which immune cells discern fungal transformations remains elusive. Here we report that the mechanosensitive ion channel Piezo1 is indispensable for recognizing fungal hyphae and triggering anti-fungal innate immune responses. Hyphae-triggered Piezo1 activation increased CLRs expression in innate immune cells by inducing the expression of C/EBPb via the Piezo1/CaMKs/CREB axis. In addition, Piezo1/CaMKs signaling activated kinases NDR1/2, which augmented NLRP3 inflammasome assembly and promoted hyphae-induced inflammation. Abolishing the yeast-to-hyphae transition dampens CLR and NLRP3 activation. Piezo1-deficient mice exhibit compromised clearance of C. albicans infection, whereas Piezo1 agonist amplifies C. albicans clearance. In addition, CaMKs, CREB or NDR1/2 inhibition exacerbates hyphae infections, like Piezo1 deficiency. Therefore, we ascertain Piezo1-mediated mechanotransduction as vital for immune surveillance and control of hyphal C. albicans, heralding Piezo1 agonist as a potential remedy for fungal infections.

2025-05-22 | GSE279850 | GEO

Piezo1 initiates host immune defense against pathogenic fungi through mechanosensory recognition of Candida albicans hyphae

2025-05-21 | GSE293094 | GEO

Transcriptional profiling of Candida albicans SC5314, hgc1Δ and cph1Δ/efg1Δ in yeast-and hyphae-inducing conditions at two time points.

Project description:Goal of this study was to determine metabolic adaptation processes in C. albicans associated to hyphal morphogenesis. Accessory to the metabolic profiling the corresponding transcriptome was investigated. To identify media-specific and general adaptation three different hyphae stimuli were used (M199 pH 7.4, Human serum and N-Aectylglucosamine) were used and compared again two respective yeast conditions (SD and M199 pH 4). Two filamentation-affected mutant strains were included - lacking either two central regulators of filamentation (cph1Δ/efg1Δ) or a downstream effector (hgc1Δ) - for the identification of specfic morphotype-dependent metabolic adaptation in repsonse to hyphae stimuli. Two different time points (90 min and 240 min after transition to test medium) were investigated to define the progression of the examined adaptation processes.

2022-10-14 | GSE202941 | GEO

Genome-wide analysis of H3K56ac across C. albicans genome in hyphae-inducing conditions

Project description:Genome-wide analysis of H3K56ac across C. albicans genome in hyphae-inducing conditions

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RNA-seq of Candida albicans in hyphae inducing conditons treated or not with nicotinamide

Project description:RNA-seq of Candida albicans in hyphae inducing conditons treated or not with nicotinamide

| PRJEB66135 | ENA

Effect of fludioxonil treatment on candida albicans SC5314

Project description:Comparison of Candida albicans SC5314 treated with fludioxonil for 30 min and untreated controls under hyphae-inducing conditions

2012-10-01 | GSE39715 | GEO

The Ndr/LATS kinase Cbk1 regulates a specific subset of Ace2 functions and suppresses the hyphae-to-yeast transition in Candida albicans

Project description:The Regulation of Ace2 and Morphogenesis (RAM) pathway is an important regulatory network in the human fungal pathogen Candida albicans. The RAM pathway’s two most well-studied components, the NDR/Lats kinase Cbk1 and its putative substrate, the transcription factor Ace2, have a wide range of phenotypes and functions. It is not clear, however, which of these functions are specifically due to the phosphorylation of Ace2 by Cbk1. To address this question, we first compared the transcriptional profiles of CBK1 and ACE2 deletion mutants. This analysis indicates that, of the large number of genes whose expression is affected by deletion of CBK1 and ACE2, only 5.5% of those genes are concordantly regulated. Our data also suggest that Ace2 directly or indirectly represses a large set of genes during hyphal morphogenesis. Second, we generated strains containing ACE2 alleles with alanine mutations at the Cbk1 phosphorylation sites. Phenotypic and transcriptional analysis of these ace2 mutants indicates that, as in Saccharomyces cerevisiae, Cbk1 regulation is important for daughter cell localization of Ace2 and cell separation during yeast phase growth. In contrast, Cbk1 phosphorylation of Ace2 plays a minor role in C. albicans yeast-to-hyphae transition. We have, however, discovered a new function for the Cbk1-Ace2 axis. Specifically, Cbk1 phosphorylation of Ace2 prevents the hyphae-to-yeast transition. To our knowledge, this is one of the first regulators of the C. albicans hyphae-to-yeast transition to be described. Finally, we present an integrated model for the role of Cbk1 in the regulation of hyphal morphogenesis in C. albicans.

2020-08-26 | GSE155450 | GEO

Microarray experiment of Candida albicans candidalysin mutants in yeast and hypha morphology

Project description:The effect of the deletion of the hyphae-associated toxon of Candida albicans, camdidalysin, on yeast and hyphae tramscriptomes was tested with different mutants. Tested strains include the wild type (SC5314), deletion mutants of candidalyin and of ECE1 (the gene encoding the polyprotein that includes candidalysin) and an ECE1 revertant strain. Each were tested in yeast- and in hypae-supporting growth conditions.

2024-01-12 | E-MTAB-13349 | biostudies-arrayexpress

Transcriptional profiling of Candida albicans SC5314, hgc1Δ and cph1Δ/efg1Δ in yeast-and hyphae-inducing conditions at two time points.

Project description:Transcriptional profiling of Candida albicans SC5314, hgc1Δ and cph1Δ/efg1Δ in yeast-and hyphae-inducing conditions at two time points.

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