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RNA-seq of Candida albicans Hda1 complex mutants (hda1∆∆, hda2∆∆ and hda3∆∆) and wildtype SN152 in yeast and hyphae inducing conditions

ABSTRACT: RNA-seq of Candida albicans Hda1 complex mutants (hda1∆∆, hda2∆∆ and hda3∆∆) and wildtype SN152 in yeast and hyphae inducing conditions

PROVIDER: PRJEB27358 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2652140.fastq.gz	Fastqsanger.gz
	ERR2652131.fastq.gz	Fastqsanger.gz
	ERR2652141.fastq.gz	Fastqsanger.gz
	ERR2652132.fastq.gz	Fastqsanger.gz
	ERR2652142.fastq.gz	Fastqsanger.gz

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RNA-seq of Candida albicans Hda1 complex mutants (hda1∆∆, hda2∆∆ and hda3∆∆) and wildtype SN152 in yeast and hyphae inducing conditions

Project description:Candida albicans is a normally commensal yeast which becomes pathogenic to human hosts under some conditions and specifically in immune compromised individuals. This contributes to a global health crisis of fungal disease. This yeast can morphologically adapt to the environment by reversibly developing hyphae which allows for traversing of tissue. Histone deacetylase 1 (Hda1) is involved in this transition as it is established that the deletion of the associated gene stunts this morphological switch. In Saccharomyces cerevisiae this protein complexes with two similarly named proteins: Hda2 and Hda3. Here we evaluate Candida albicans strains with mutations in the genes that code for these proteins. The strains are grown in yeast (YPD 30 °C) and hyphae (RPMI 37 °C) -inducing conditions for 90 minutes prior to RNA extraction. After alignment by HISAT2, the homozygous mutants are then individually compared to wildtype (matching growth conditions) using DESeq2.

2023-03-01 | E-MTAB-6920 | biostudies-arrayexpress

Transcriptional profiling of Candida albicans SC5314, hgc1Δ and cph1Δ/efg1Δ in yeast-and hyphae-inducing conditions at two time points.

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Genome-wide analysis of H3K56ac across C. albicans genome in hyphae-inducing conditions

Project description:Genome-wide analysis of H3K56ac across C. albicans genome in hyphae-inducing conditions

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RNA-seq of Candida albicans in hyphae inducing conditons treated or not with nicotinamide

Project description:RNA-seq of Candida albicans in hyphae inducing conditons treated or not with nicotinamide

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Effect of fludioxonil treatment on candida albicans SC5314

Project description:Comparison of Candida albicans SC5314 treated with fludioxonil for 30 min and untreated controls under hyphae-inducing conditions

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The Ndr/LATS kinase Cbk1 regulates a specific subset of Ace2 functions and suppresses the hyphae-to-yeast transition in Candida albicans

Project description:The Regulation of Ace2 and Morphogenesis (RAM) pathway is an important regulatory network in the human fungal pathogen Candida albicans. The RAM pathway’s two most well-studied components, the NDR/Lats kinase Cbk1 and its putative substrate, the transcription factor Ace2, have a wide range of phenotypes and functions. It is not clear, however, which of these functions are specifically due to the phosphorylation of Ace2 by Cbk1. To address this question, we first compared the transcriptional profiles of CBK1 and ACE2 deletion mutants. This analysis indicates that, of the large number of genes whose expression is affected by deletion of CBK1 and ACE2, only 5.5% of those genes are concordantly regulated. Our data also suggest that Ace2 directly or indirectly represses a large set of genes during hyphal morphogenesis. Second, we generated strains containing ACE2 alleles with alanine mutations at the Cbk1 phosphorylation sites. Phenotypic and transcriptional analysis of these ace2 mutants indicates that, as in Saccharomyces cerevisiae, Cbk1 regulation is important for daughter cell localization of Ace2 and cell separation during yeast phase growth. In contrast, Cbk1 phosphorylation of Ace2 plays a minor role in C. albicans yeast-to-hyphae transition. We have, however, discovered a new function for the Cbk1-Ace2 axis. Specifically, Cbk1 phosphorylation of Ace2 prevents the hyphae-to-yeast transition. To our knowledge, this is one of the first regulators of the C. albicans hyphae-to-yeast transition to be described. Finally, we present an integrated model for the role of Cbk1 in the regulation of hyphal morphogenesis in C. albicans.

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Effect of fludioxonil treatment on candida albicans SC5314

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RNA-seq data for bone-marrow-derived macrophages from wildtype and JNK1 knockout mice stimulated with yeast form Candida albicans

Project description:mRNA expression profile for BMDMs from wildtype and JNK1 knockout mice stimulated with yeast form Candida albicans

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