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Genome sequences of African field-isolates of rice blast fungus

ABSTRACT: Genomic DNA was sequenced from rice blast fungus collected from different locations in Africa.

PROVIDER: PRJEB27419 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR14126201_1.fastq.gz	Fastqsanger.gz
	ERR14126201_2.fastq.gz	Fastqsanger.gz
	ERR14112733_1.fastq.gz	Fastqsanger.gz
	ERR14112733_2.fastq.gz	Fastqsanger.gz

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Project description:Fourth leaves of rice seedlings (4.5 leaf stage) grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray.

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Gene expression in rice leaves (Pia) in response to inoculation with rice blast fungus

Project description:Fourth leaves of rice seedlings (4.5 leaf stage) grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray.

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RRM Transcription Factors Interact with NLRs and Regulate Broad-Spectrum Blast Resistance in Rice

Project description:Nucleotide-binding site leucine-rich repeat (NLR) receptors perceive pathogen effectors and trigger plant immunity. However, the mechanisms underlying NLR-triggered defense responses remain obscure. The recently discovered Pigm locus in rice encodes a cluster of NLRs, including PigmR, which confers broad-spectrum resistance to blast fungus. Here, we identify PIBP1 (PigmR-INTERACTING and BLAST RESISTANCE PROTEIN 1), an RRM (RNA-recognition motif) protein that specifically interacts with PigmR and other similar NLRs to trigger blast resistance. PigmR-promoted nuclear accumulation of PIBP1 ensures full blast resistance. We find that PIBP1 and a homolog, Os06 g02240, bind DNA and function as unconventional transcription factors at the promoters of the defense genes OsWAK14 and OsPAL1, activating their expression. Knockout of PIBP1 and Os06g02240 greatly attenuated blast resistance. Collectively, our study discovers previously unappreciated RRM transcription factors that directly interact with NLRs to activate plant defense, establishing a direct link between transcriptional activation of immune responses with NLR-mediated pathogen perception

2019-08-04 | GSE118355 | GEO

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