Project description:β-Mannans are plant cell wall polysaccharides that are commonly found in human diets. However, a mechanistic understanding into the key populations that degrade this glycan is absent, especially for the dominant Firmicutes phylum. Here, we show that the prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans. We combine multi-“omic” analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins that are involved in β-mannan capturing, importation, de-branching and degradation into monosaccharides. In mixed cultures, R. intestinalis shares the available β-mannan with Bacteroides ovatus, demonstrating that the apparatus allows coexistence in a competitive environment. In murine experiments, β-mannan selectively promotes beneficial gut bacteria, exemplified by increased R. intestinalis, and reduction of mucus-degraders. Our findings highlight that R. intestinalis is a primary degrader of this dietary fiber and that this metabolic capacity could be exploited to selectively promote key members of the healthy microbiota using β-mannan-based therapeutic interventions.

2019-02-21 | PXD012448 | Pride

Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model

Project description:Background: Humans with metabolic and inflammatory diseases frequently harbor lower levels of butyrate-producing bacteria in their gut. However, it is not known whether variation in the levels of these organisms is causally linked with disease development and whether diet modifies the impact of these bacteria on health. Results: We use germ-free apolipoprotein E-deficient mice colonized with synthetic microbial communities that differ in their capacity to generate butyrate to demonstrate that Roseburia intestinalis interacts with dietary components to (i) impact gene expression in the intestine, directing metabolism away from glycolysis and toward fatty acid utilization, (ii) improve intestinal barrier function, (iii) lower systemic inflammation and (iv) ameliorate atherosclerosis. Furthermore, intestinal administration of butyrate improves gut barrier function and reduces atherosclerosis development. Conclusions: Altogether, our results illustrate how modifiable diet-by-microbiota interactions impact cardiovascular disease, and suggest that interventions aimed at increasing the representation of butyrate-producing bacteria may provide protection against atherosclerosis.

2018-08-29 | GSE119141 | GEO

Proteomics for Roseburia intestinalis and Parabacteroides merdae exposed to isosteviol and duloxetine.

Project description:The objective of this project was to identify changes in protein expression in R. intestinalis and P. merdae as a response to the treatment with the antidepressant duloxetine, the sweetener isosteviol and a combination of both. 18 samples before and after treatment were collected for each of the two species under anaerobic conditions. The species were inoculated from frozen stock cultures, three replicates each, into mGAM medium and passaged twice over night. Passage two was inoculated into 60 ml (120 ml for R. intestinalis) medium and pre-exposure0 samples were collected directly hereafter (three per species, one sample per replicate). The second pre-exposure samples were collected upon reaching exponential growth, directly prior to splitting each replicate into four equal sized batches, one per condition: Mock (DMSO, 0.2 %), 50 µM duloxetine, 50 µM isosteviol and a combination of both, 50 µM each. This results in 12 treatment samples, three per treatment, whereas each of the replicates can be traced back to one preculture. Bacteria were grown in the presence of the compounds at 37˚C for four hours, before final sample collection. Pre-exposure samples were collected as controls to monitor changes in protein expression in different growth stages.

2026-04-30 | PXD045140 | Pride

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