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RNA-seq of E14Tg2a cells upon Zic3 knockdown after day1 and day2 of differentiation into epiblast-like stem cells

ABSTRACT: RNA-seq of E14Tg2a cells upon Zic3 knockdown after day1 and day2 of differentiation into epiblast-like stem cells

PROVIDER: PRJEB28630 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2791300_1.fastq.gz	Fastqsanger.gz
	ERR2791300_2.fastq.gz	Fastqsanger.gz
	ERR2791301_1.fastq.gz	Fastqsanger.gz
	ERR2791301_2.fastq.gz	Fastqsanger.gz
	ERR2791292_1.fastq.gz	Fastqsanger.gz

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RNA-seq of E14Tg2a cells upon Zic3 knockdown after day1 and day2 of differentiation into epiblast-like stem cells

Project description:Identification of Zic3-dependent transcriptome at d1EpiLC and d2EpiLC. Gene expression was studied in the presence or absence of Zic3 (RNAi) in the Rex1GFPd2 mouse embryonic stem cell line (parental line; E14Tg2a) in d1- and d2-EpiLC differentiated states.

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Project description:In order to better understand the molecular basis for the heart defects seen in Zic3 null and epiblast CKO embryos, we investigated whether complete or epiblast-specific deletion of Zic3 would impact later embryonic heart development at the transcriptional level by whole genome expression microarray. The whole heart was carefully dissected out from 15.5 dpc Zic3 +/y, Zic3 flox/y, Zic3 flox/y; Sox2-cre, and Zic3 -/y embryos, total RNAs were extracted and purified using RNeasy Mini Kit (QIAGEN). Spectrophotometry (NanoDrop-1000 Spectrophotometer, Thermo Fisher Scientific) and microfluidic electrophoresis (Experion Automated Electrophoresis System, Bio-Rad Laboratories) were used for RNA quality control. In vitro transcription was performed using Illumina TotalPrep RNA Amplification Kit (Applied Biosystems/Ambion). cRNAs were hybridized onto Illumina MouseWG-6 v2.0 Expression BeadChips (Illumina) per manufacturer’s instructions.

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Project description:RNA-seq of E14Tg2a cells upon Zic3 knockdown after day1 and day2 of differentiation into epiblast-like stem cells

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