Project description:The microalga Coccomyxa subellipsoidea C-169 possesses some features that may be valuable for lipid production, and, as demonstrated in this study, can be greatly induced to produce a high amount of fatty acid by CO2 supplementation. Here we have compared the transcriptome of air group (AG, cells cultured under 0.04% CO2) and CO2-supplemented group (CG, cells cultured under 2% CO2), and found that dramatic and collaborative regulation in central metabolic pathways as well as biochemical processes occured in response to CO2 supplementation. This study gains a broad understanding of how CO2 stress regulates gene expression and eventually reveals a fine-tuned strategy adopted by C-169 to sustain rapid cell growth and lipid production, which will be helpful for the implementation of biofuels production from oleaginous microalgae. Transcriptomic profiles of Coccomyxa subellipsoidea C-169 cultured for 4 days under two CO2 levels (0.04% and 2%, v/v) were generated by digital gene expression (DGE) analysis, in triplicate, using Illumina Hiseq2000.
Project description:The microalga Coccomyxa subellipsoidea C-169 possesses some features that may be valuable for lipid production, and, as demonstrated in this study, can be greatly induced to produce a high amount of fatty acid by CO2 supplementation. Here we have compared the transcriptome of air group (AG, cells cultured under 0.04% CO2) and CO2-supplemented group (CG, cells cultured under 2% CO2), and found that dramatic and collaborative regulation in central metabolic pathways as well as biochemical processes occured in response to CO2 supplementation. This study gains a broad understanding of how CO2 stress regulates gene expression and eventually reveals a fine-tuned strategy adopted by C-169 to sustain rapid cell growth and lipid production, which will be helpful for the implementation of biofuels production from oleaginous microalgae.
Project description:•Carnivorous plants from the order Caryophyllales co-opted plant phytohormones from the group of jasmonates for regulation of digestive enzyme activities. But not all genera of carnivorous plants are evenly explored and the digestive physiology of the Australian carnivorous rainbow plants of the genus Byblis (order: Lamiales) is only very poorly understood. •Here, we investigated the composition of digestive enzymes in the secreted fluid of Byblis filifolia using LC/MS, measured enzyme activities and analysed the phytohormone tissue level after experimental feeding on fruit flies and coronatine application. •Several hydrolytic digestive enzymes were identified in the secreted digestive fluid which were clearly upregulated in the presence of insect prey. But in contrast to sundew plant Drosera capensis, endogenous jasmonate did not accumulate and coronatine, a molecular mimic of jasmonates, was not able to trigger enzyme secretion. •Our results document that carnivorous plant B. filifolia is fully carnivorous with their own digestive enzyme repertoire. However in contrast to carnivorous genera from the order Caryophyllales, they are not under the control of jasmonates. This indicates that jasmonates have not been co-opted for regulation of digestive enzyme activity repeatedly during evolution of carnivorous plants.
Project description:Transcriptome analysis reveals global regulation in response to CO2 supplementation in oleaginous microalga Coccomyxa subellipsoidea C-169
