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3' mRNA-seq of neurites and soma isolated from mESC-derived neurons

ABSTRACT: 3' mRNA-seq of neurites and soma isolated from mESC-derived neurons

PROVIDER: PRJEB28860 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2817191.fastq.gz	Fastqsanger.gz
	ERR2817192.fastq.gz	Fastqsanger.gz
	ERR2817193.fastq.gz	Fastqsanger.gz
	ERR2817194.fastq.gz	Fastqsanger.gz
	ERR2817195.fastq.gz	Fastqsanger.gz

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3' mRNA-seq of neurites and soma isolated from mESC-derived neurons

Project description:3' mRNA-seq was performed with QuantSeq 3' mRNA-Seq kit (Lexogen 015) according to the manufacturer’s recommendations. 3' mRNA-seq was done in biological triplicates (soma) or duplicates (neurites), using 260 ng of total RNA from neurites or soma of mESC-derived neurons per sample. Libraries were pooled and sequenced on Illumina NextSeq 500 system with a single-end 150-cycle run.

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