Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Anaerobic fungi ITS1 HiSeq Method Assessment

ABSTRACT: Assessment of the Accuracy of Anaerobic Fungal ITS1 Based Barcoded Amplicon Sequencing for Community Composition Analysis

PROVIDER: PRJEB29131 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2835040_1.fastq.gz	Fastqsanger.gz
	ERR2835040_2.fastq.gz	Fastqsanger.gz
	ERR2835041_1.fastq.gz	Fastqsanger.gz
	ERR2835041_2.fastq.gz	Fastqsanger.gz
	ERR2835042_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Oryza sativa ITS1 sequencing

Project description:Oryza sativa ITS1 sequencing

| PRJNA533998 | ENA

Yulongxueshan soil samples-ITS1

Project description:Yulongxueshan soil samples-ITS1 Metagenome

| PRJNA957854 | ENA

ITS1 of Longya lily

Project description:ITS1 of Longya lily Raw sequence reads

| PRJNA752683 | ENA

16S and ITS1 sequences Imbalance-P

Project description:16S and ITS1 sequences Imbalance-P Raw sequence reads

| PRJNA796750 | ENA

Lignin deconstruction by anaerobic fungi.

Project description:Lignocellulose forms plant cell walls, and its three constituent polymers, cellulose, hemicellulose and lignin, represent the largest renewable organic carbon pool in the terrestrial biosphere. Insights into biological lignocellulose deconstruction inform understandings of global carbon sequestration dynamics and provide inspiration for biotechnologies seeking to address the current climate crisis by producing renewable chemicals from plant biomass. Organisms in diverse environments disassemble lignocellulose, and carbohydrate degradation processes are well defined, but biological lignin deconstruction is described only in aerobic systems. It is currently unclear whether anaerobic lignin deconstruction is impossible because of biochemical constraints or, alternatively, has not yet been measured. We applied whole cell-wall nuclear magnetic resonance, gel-permeation chromatography and transcriptome sequencing to interrogate the apparent paradox that anaerobic fungi (Neocallimastigomycetes), well-documented lignocellulose degradation specialists, are unable to modify lignin. We find that Neocallimastigomycetes anaerobically break chemical bonds in grass and hardwood lignins, and we further associate upregulated gene products with the observed lignocellulose deconstruction. These findings alter perceptions of lignin deconstruction by anaerobes and provide opportunities to advance decarbonization biotechnologies that depend on depolymerizing lignocellulose.

| S-EPMC10066034 | biostudies-literature

ITS1 & ITS2 amplicons of soil sample in 2018

Project description:ITS1 & ITS2 amplicons of soil sample in 2018

| PRJEB67552 | ENA

Anaerobic Fungi: Past, Present, and Future.

Project description:Anaerobic fungi (AF) play an essential role in feed conversion due to their potent fiber degrading enzymes and invasive growth. Much has been learned about this unusual fungal phylum since the paradigm shifting work of Colin Orpin in the 1970s, when he characterized the first AF. Molecular approaches targeting specific phylogenetic marker genes have facilitated taxonomic classification of AF, which had been previously been complicated by the complex life cycles and associated morphologies. Although we now have a much better understanding of their diversity, it is believed that there are still numerous genera of AF that remain to be described in gut ecosystems. Recent marker-gene based studies have shown that fungal diversity in the herbivore gut is much like the bacterial population, driven by host phylogeny, host genetics and diet. Since AF are major contributors to the degradation of plant material ingested by the host animal, it is understandable that there has been great interest in exploring the enzymatic repertoire of these microorganisms in order to establish a better understanding of how AF, and their enzymes, can be used to improve host health and performance, while simultaneously reducing the ecological footprint of the livestock industry. A detailed understanding of AF and their interaction with other gut microbes as well as the host animal is essential, especially when production of affordable high-quality protein and other animal-based products needs to meet the demands of an increasing human population. Such a mechanistic understanding, leading to more sustainable livestock practices, will be possible with recently developed -omics technologies that have already provided first insights into the different contributions of the fungal and bacterial population in the rumen during plant cell wall hydrolysis.

| S-EPMC7609409 | biostudies-literature

Brassica napus soil metabarcoding ADNr 16S & ITS1

Project description:Brassica napus soil metabarcoding ADNr 16S & ITS1 Raw sequence reads

| PRJNA854370 | ENA

Fungal ITS1 of beech leaves (Fagus Sylvatica)

Project description:Fungal ITS1 of beech leaves (Fagus Sylvatica) Raw sequence reads

| PRJNA699462 | ENA

Phytophthora ITS1 amplicon Illumina MiSeq sequencing.

Project description:Phytophthora ITS1 amplicon Illumina MiSeq sequencing in tree nurseries and wider environment.

| PRJEB76241 | ENA