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RPTEC/TERT1

ABSTRACT: RPTEC/TERT1 at baseline using different protocols

PROVIDER: PRJEB29471 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2865440_1.fastq.gz	Fastqsanger.gz
	ERR2865440_2.fastq.gz	Fastqsanger.gz
	ERR2865450_1.fastq.gz	Fastqsanger.gz
	ERR2865450_2.fastq.gz	Fastqsanger.gz
	ERR2865441_1.fastq.gz	Fastqsanger.gz

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miRNA expression profiling of hTERT-immortalized renal proximal tubule epithelial (RPTEC/TERT1) cell treated with mesenchymal stem cell-conditioned miedium (BMSC-CM) on polyacrylamide (PAA) gel platform

Project description:Chronic kidney disease (CKD) is a progressive condition characterized by sustained alterations in kidney structure and function. Long-term kidney fibrosis is marked by glomerulosclerosis, vascular sclerosis, and tubulointerstitial fibrosis. Therefore we investigated the therapeutic potential of BMSC-CM on RPTEC/TERT1 in a fibrotic environment. Using PAA gel platforms, we mimicked the stiffness typical of chronic kidney disease patients' kidneys.

2023-12-20 | GSE250271 | GEO

Exposure of EFPIA compounds to RPTEC/TERT1 cell lines

Project description:Exposure of EFPIA compounds to RPTEC/TERT1 cell lines

| PRJEB55273 | ENA

Tacrolimus TK173 RPTEC

Project description:Confluent cultures of a human renal fibroblast cell line (TK-173) and a human renal proximal tubule epithelial cell line (RPTEC/TERT1) were treated with 10 micromolar tacrolimus (FK-506) for one and three days.

2012-01-01 | E-MTAB-778 | biostudies-arrayexpress

Microarray mRNA data of RPTEC/TERT1 cells exposed to nephrotoxic chemicals ochratoxin A and potassium bromate

Project description:Human renal proximal tubule cells (RPTEC/TERT1) were exposed to one of two nephrotoxic chemicals, ochratoxin A (OA) or potassium bromate (KB), or a vehicle control (CT). The exposure regime was a repeated dosing every 24h for 5 days followed by chemical washout and 3 days of recovery with medium renewal every 24h. Total experimental duration was 8 days. Cell lysates were prepared for microarray analysis after 1 day, 3 days and 5 days of exposure, and after 3 days of recovery (day 8).

2018-09-16 | E-MTAB-7048 | biostudies-arrayexpress

High throughput targeted RNA sequencing on RPTEC/TERT1 cell line exposed to multiple concentration levels of cyclosporine A at various different time points

Project description:This experiment was carried out to investigate the time dynamics of the proximal tubule cells upon the exposure of cyclosporine A in multiple severity scenario by varying the concentration. The RPTEC/TERT1 cells which are immortalized human proximal tubule cells were differentiated for 14 days to reach the optimal maturity where the cells express the proximal tubule markers and transporter similarly to human proximal tubule cells. The cells were then exposed to cyclosporine A at multiple concentration levels ranging from 0-40 uM for 4, 8,16, 24,48, and 72 hours. The cells were lysed and the lysates were collected for high throughput targeted RNA-seq with the human whole genome library. The gene expression profiles were analyzed to identify the modulated responses of the cells. This is part of the TransQST project.

2023-06-10 | E-MTAB-11553 | biostudies-arrayexpress

A comparison between novel 3D human renal proximal tubule organoids and 2D monolayer cells utilizing RPTEC-TERT1 cells

Project description:A comparison between novel 3D human renal proximal tubule organoids and 2D monolayer cells utilizing RPTEC-TERT1 cells

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High throughput targeted RNA sequencing on RPTEC/TERT1 cell line exposed to multiple concentration levels of cyclosporine A at various different time points

Project description:High throughput targeted RNA sequencing on RPTEC/TERT1 cell line exposed to multiple concentration levels of cyclosporine A at various different time points

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Mechanobiological proximal tubular defects in ARC syndrome: A VPS33B CRISPR knockout study

Project description:ARC syndrome (arthrogryposis-renal dysfunction-cholestasis) is a rare autosomal recessive multisystem disorder affecting the kidneys. The disease is caused by mutations in either VPS33B or VIPAS39. ARC syndrome is currently incurable, with patients rarely surviving beyond their first year of life. The renal component of this disorder is characterised by proximal tubular dysfunction. Here, a proximal tubular cell line, RPTEC-TERT1, was CRISPR-edited to knock out (KO) VPS33B expression. Characterisation of VPS33B-KO cells was performed using brightfield imaging, immunostaining, RNA sequencing, and cell detachment assays. The VPS33B-KO RPTEC-TERT1 cells demonstrated a 'peeling' phenotype and altered cell adhesion. This, along with altered transcription of genes associated with cell adhesion, suggests that VPS33B KO results in cell-matrix attachment defects. These findings provide the first insights into the cause of proximal tubular dysfunction in ARC syndrome.

2025-06-23 | GSE298726 | GEO

Bulk RNA-seq of irradiated RPTEC cells

Project description:RPTEC cells were cultured and irradiated to induce cellular senescence. Bulk RNA-seq was performed to observe the transcriptional response.

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Project description:The kidneys are essential for eliminating drugs and chemicals from the human body, with renal epithelial cells being particularly vulnerable to damage caused by xenobiotics and their metabolites. Predicting renal toxicity before clinical trials remains a pressing challenge, necessitating more predictive in vitro models. However, the abundance of commercially available renal proximal tubule epithelial cell (RPTEC) sources complicates the selection of the most predictive cell types. This study compared RPTEC sources, including primary cells (Lonza) and various RPTEC lines, such as ciPTECs (Cell4Pharma), TERT1/RPTECs (ATCC), and HEK293 (GenoMembrane), alongside HepG2 cells for specificity comparison. We tested these cells' responses to 12 drugs to evaluate their predictive capabilities. Cells were cultured in 96-well or 384-well plates and exposed to drugs for 72 hours at concentrations ranging from 0.3 to 300 µM. The CellTiterGlo® assay measured cell viability, and transcriptome data from unexposed cells analyzed gene expression profiles using the TempO-seq® S1500+ panel. Cell viability data determined points of departure (PODs), compared to human serum Cmax,free values to assess safety margins. Gene expression showed primary kidney cells closely matched the human kidney medulla, followed by TERT1 and ciPTEC lines, with HEK lines showing the least correlation. This study highlights differences among RPTEC sources and their utility in drug safety studies for the renal proximal tubule, emphasizing unique gene expression patterns related to metabolism, ion transport, and cell development, which are vital for improving predictive models in renal toxicity assessment.

2025-04-02 | GSE268877 | GEO

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