Project description:Gene transcription in epididymal fat pads was investigated in an F2 cross of 129S6 x Balb/c mice using Illumina gene expression arrays. Expression data was determined in 5 months old male mice fed a carbohydrate diet. Genotyping data for the F2 offspring used in this study can be found in the file E-MTAB-395.additional.zip under the Browse all available files link (file mouse-f2-chd-genotype.txt added 8th April 2014).
Project description:Anterior pituitary glands were isolated from 21 week old male rats treated for 12 weeks with the synthetic estrogen diethylstilbestrol (DES). 43 COPxACI F2 animals were selected based on pituitary weight phenotypic extremes: 22 highest and 21 lowest pituitary weights in our F2 population. Expression profiles were determined using Affymetrix Rat Genome 230 v. 2.0 arrays. In conjunction with genomewide genotypes available for these 43 animals, linkage analysis was done using mRNA abundance of each transcript on the array as a separate quantitative phenotype. These arrays provide a resource for expression QTL (eQTL) mapping on a genomewide level in the pituitary gland. Keywords: Estrogen Response
Project description:Anterior pituitary glands were isolated from 21 week old male rats treated for 12 weeks with the synthetic estrogen diethylstilbestrol (DES). 43 COPxACI F2 animals were selected based on pituitary weight phenotypic extremes: 22 highest and 21 lowest pituitary weights in our F2 population. Expression profiles were determined using Affymetrix Rat Genome 230 v. 2.0 arrays. In conjunction with genomewide genotypes available for these 43 animals, linkage analysis was done using mRNA abundance of each transcript on the array as a separate quantitative phenotype. These arrays provide a resource for expression QTL (eQTL) mapping on a genomewide level in the pituitary gland. Experiment Overall Design: 43 samples from anterior pituitary gland were analyzed. COPxACI F2 male animals were treated with DES for 12 weeks, starting at 9 weeks of age. Pituitary glands were harvested and weighed at sacrifice (21 weeks of age). The 43 samples were selected for pituitary weight phenotypic extremes: 22 highest and 21 lowest pituitary weights in our F2 population. Genomewide genotypes are available for these animals and in conjunction with the array data, genomewide expression QTL (eQTL) mapping was done.
Project description:Abiotic stress triggered by Drought or soil salinity impacts negatively on crop production. To better understand amaranth responses to those environmental stresses, transcriptomics approaches were applied to investigate changes at molecular level in leaves of two Amaranthus species: A. hybridus, a wild species that grows in saline soils and A. hypochondriacus, the most cultivated species for grain production. Under control conditions, A. hybridus showed up-regulation of organic hydroxyl and sulfur compound processes, while A. hypochondriacus showed up-regulation of genes related to photosynthetic process, amino acids, fatty acids, and secondary metabolism. Under salt stress, significantly fewer DEGs were observed in A. hybridus, interestingly those were related to phosphonate and phosphinate metabolism, phosphorous mobilization, and steroid biosynthesis. In contrast, A. hypochondriacus response to salt stress was characterized by starch, sucrose, and trehalose metabolism, porphyrin, monoterpenoid and organonitrogen compounds biosynthetic process.Under water deficit, both species showed similar transcriptional response by up-regulation of LEAs, dehydrins, and genes related to the biosynthesis of Raffinose Family Oligosaccharides. A. hypochondriacus better adaptation to grow under drought and salt stress could be due to its fast response observed by the up-regulation of MAPK signaling pathways, ABC transporters, and production of defense compounds such as glucosinolates. The common response in both amaranth species to both type of stresses was observed by modulation of secondary metabolism, which was directed towards cell wall modification, cuticle and wax metabolism, and the production of antimicrobial and antifungal metabolites