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RNAseq of 4 day old etiolated Arabidopsis thaliana Ler and gin2-1 seedlings grown in darkness at 18C

ABSTRACT: RNAseq of 4 day old etiolated Arabidopsis thaliana Ler and gin2-1 seedlings grown in darkness at 18C

PROVIDER: PRJEB31168 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3148731_1.fastq.gz	Fastqsanger.gz
	ERR3148731_2.fastq.gz	Fastqsanger.gz
	ERR3148732_1.fastq.gz	Fastqsanger.gz
	ERR3148732_2.fastq.gz	Fastqsanger.gz
	ERR3148733_1.fastq.gz	Fastqsanger.gz

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RNAseq of 4 day old etiolated Arabidopsis thaliana Ler and gin2-1 seedlings grown in darkness at 18C

Project description:Post-germination, emerging seedlings rely on storage reserves to fuel cellular metabolism until photoautotrophic growth is established. Seed reserves can also sustain growth when seedlings are occluded from light, by for instance, soil or debris, which delays the switch to photosynthesis-driven growth. This study has shown that HEXOKINASE 1 (HXK1) supports early seedling growth, particularly during periods of prolonged darkness or low light. HXK1 is an evolutionarily conserved enzyme that catalyzes the first glycolytic step in cellular respiration for energy production. We show that HXK1 also has a broader function in nutrient resource management, balancing the transcriptional control of energy generating vs demanding processes with the seedling internal carbon status.

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Project description:Transcriptional profiling of Arabidopsis thaliana Ler wildtype and eid3 (empfindlicher im dunkelroten Licht 3) mutant seedlings in darkness and 45 min after a red-light pulse. Arabidopsis thaliana Ler wildtype and eid3 mutant seedlings were grown on 1/2 MS Agar plates covered with filter paper for 4 days in darkness after induction of germination with 2 h red light. Samples were either treated with 2 min red light (30 µmol/m2s) or kept in darkness and harvested after additional 45 min in darkness. 3 biological replicas were used for each of the 4 experimental conditions.

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