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ChIPseq of enterocytes of the jejunum from transgene C57BL/6 mice harboring a CKIαΔgut knock-out

ABSTRACT: ChIPseq of enterocytes of the jejunum from transgene C57BL/6 mice harboring a CKIαΔgut knock-out

PROVIDER: PRJEB32078 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3266322.fastq.gz	Fastqsanger.gz
	ERR3266323.fastq.gz	Fastqsanger.gz
	ERR3266324.fastq.gz	Fastqsanger.gz
	ERR3266325.fastq.gz	Fastqsanger.gz
	ERR3266326.fastq.gz	Fastqsanger.gz

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ChIPseq of enterocytes of the jejunum from transgene C57BL/6 mice harboring a CKIαΔgut knock-out

Project description:ChIPseq of enterocytes of the jejunum from transgene C57BL/6 mice to assess the effects of a CKIαΔgut knock-out in the jejunum and a CKIαΔgut/p53 double knock-out. In addition, enterocytes expressing a mutant p53 (R172H) in combination with CKIαΔgut knockout were analyzed.

2020-02-25 | E-MTAB-7858 | biostudies-arrayexpress

RNAseq of enterocytes of the jejunum and Ileum from transgene C57BL/6 mice harboring a CKIαΔgut knock-out

Project description:RNAseq of enterocytes of the jejunum and Ileum from transgene C57BL/6 mice to assess the effects of a CKIα knock-out in combination with expression of a p53 mutant protein (R172H ) compared to a double knock-out of CKIα and p53. In addition, a group of mice exhibiting a CKIα knock-out and mutant p53 (R172H) was treated with gallic acid and compared to untreated mice (Jejunum only).

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RNAseq of enterocytes of the jejunum and Ileum from transgene C57BL/6 mice harboring a CKIαΔgut knock-out

Project description:RNAseq of enterocytes of the jejunum and Ileum from transgene C57BL/6 mice harboring a CKIαΔgut knock-out

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Effect of depletion of Drosophila Hepatocyte Nuclear Factor 4 (dHNF4) in enterocytes on gene expression in the adult midgut

Project description:The main objective of the sudy was to characterize the functions of Drosophila Hepatocyte Nuclear Factor 4 (dHNF4) in midgut enterocytes. For this, we expressed a UAS-dHNF4 RNAi transgene under the control of mex-GAL4, which is active specifically in midgut enterocytes. We then collected adult male midguts, extracted total RNA and performed mRNA-seq. Our data demonstrate that dHNF4 acts as a master regulator of intestinal lipid metabolism in Drosophila.

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Project description:RNAseq of enterocytes of the jejunum and Ileum from transgene C57BL/6 mice harboring a CKIÎ±Î”gut knock-out

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Transcription profiling of mouse intestinal epithelial spheroids cultured as stem cells, wound-associated epithelial cells or enterocytes

Project description:Intestinal epithelial spheroids were isolated from mouse jejunum as described in our previous publication (Miyoshi and Stappenbeck. In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture. Nature Protocols. 2013. PMID: 24232249) and then grown in 50% L-WRN conditioned medium (for stem cells) or differentiation medium containing dmPGE2 (for wound-associated epithelial cells) or differentiation medium containing EP4 inhibitor (for enterocytes). Transcription profiling was performed to gain insight into the characteristics distinguishing these cell types.

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Chromosome-wide DNA unmethylome profiling examining human chromosomes 2, 9, 19 and mouse chromosomes 1, 9, 19

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chipseq

Project description:chipseq data

| PRJEB39779 | ENA