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RNA-seq of circadian timeseries sampling (LL2-3) of 13-14 day old Arabidopsis thaliana Col-0 (24 h to 68 h, sampled every 4 h)

ABSTRACT: RNA-seq of circadian timeseries sampling (LL2-3) of 13-14 day old Arabidopsis thaliana Col-0 (24 h to 68 h, sampled every 4 h)

PROVIDER: PRJEB32446 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3310290_1.fastq.gz	Fastqsanger.gz
	ERR3310290_2.fastq.gz	Fastqsanger.gz
	ERR3310300_1.fastq.gz	Fastqsanger.gz
	ERR3310300_2.fastq.gz	Fastqsanger.gz
	ERR3310291_1.fastq.gz	Fastqsanger.gz

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RNA-seq of circadian timeseries sampling (LL2-3) of 13-14 day old Arabidopsis thaliana Col-0 (24 h to 68 h, sampled every 4 h)

Project description:The experiment was designed to get high throughput mRNA data (for gene expression and alternative splicing analysis) of A. thaliana 13-14 day old plants, over the course of two days after 24h of transfer to free running (circadian) conditions. Briefly, plants were grown in MS plates (30 plants per plate / 2 plates per timepoint) for 11 days in LD 12 h : 12 h conditions (50umoles /m2 /s) and 22C. On day 12 plants were transferred to LL conditions. Sampling was performed at 4 h intervals from 24 h after transfer to LL till 68 h. Samples were flash frozen in liquid nitrogen and total RNA was extracted using the Trizol method. RNA quality was chequed by gel and libraries were constructed using the TruSeq RNA Library Prep Kit v2. QC was performed on an Agilent BioAnalyzer 2100. Libraries were then pooled and sequenced on an Ilumina HiSeq 2500 by INDEAR, Rosario, Argentina.

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