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RNA-seq of whole mouse placentas at E10.5 comparing C57Bl/6 male conceptuses that had undergone blastocyst transfer to non-transferred C57Bl/6 male conceptuses

ABSTRACT: RNA-seq of whole mouse placentas at E10.5 comparing C57Bl/6 male conceptuses that had undergone blastocyst transfer to non-transferred C57Bl/6 male conceptuses

PROVIDER: PRJEB32962 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3366050.fastq.gz	Fastqsanger.gz
	ERR3366051.fastq.gz	Fastqsanger.gz
	ERR3366052.fastq.gz	Fastqsanger.gz
	ERR3366043.fastq.gz	Fastqsanger.gz
	ERR3366053.fastq.gz	Fastqsanger.gz

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RNA-seq of whole mouse placentas at E10.5 comparing C57Bl/6 male conceptuses that had undergone blastocyst transfer to non-transferred C57Bl/6 male conceptuses

Project description:The RNA-seq experiment was designed to determine the effects of blastocyst transfer on the transcriptome of whole placentas at embryonic day (E) 10.5. C57Bl/6 blastocysts that were from natural matings without superovulation were transferred into the uteri of pseudopregnant B6D2F1 females (at gestational day 2.5 equivalent). RNA libraries were prepared from whole male placentas at E10.5 (N = 4 placentas) from the transfer group alongside RNA libraries from control placentas from C57Bl/6 conceptuses derived from natural matings and did not undergo the transfer process (N = 4 placentas). Libraries were muliplexed and pooled for sequencing on the Illumina NextSeq500 platform with 75-base-pair single-end reads. Sequencing was performed in duplicate to provide at least 18 million reads per sample, and 1% PhiX Control (Illumina) spike-in was used. Quality control of Fastq files was performed using FastQC and fastq_screen. Sequences were trimmed with Trim Galore!, and alinged to GRCm38 mouse genome using STAR aligner.Alignments were processed using custom ClusterFlow (v0.5dev) pipelines and assessed using MultiQC (0.9.dev0). Gene quantification was determined with HTSeq-Counts (v0.6.1p1). Additional quality control was performed with rRNA and mtRNA counts script, feature counts (v 1.5.0-p2) and qualimap (v2.2). Differential gene expression was performed with DESeq2 package (v1.16.1, R v3.4.0). Read counts were normalised on the estimated size factors.

2019-12-31 | E-MTAB-8036 | biostudies-arrayexpress

E-MTAB-8036

Project description:RNA-seq of whole mouse placentas at E10.5 comparing C57Bl/6 male conceptuses that had undergone blastocyst transfer to non-transferred C57Bl/6 male conceptuses

2019-09-09 | E-MTAB-8036 | ExpressionAtlas

Skeletal muscle from male C57BL/6J mice at 3-month-old or 24-month-old

Project description:Skeletal muscle from male C57BL/6J mice at 3-month-old or 24-month-old

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Females translate male mRNA transferred during mating

Project description:Although the presence of RNA in seminal fluid appears to be a conserved feature of male ejaculates, it is unknown whether this RNA is functional within females. Here, we develop an experimental proteomic method called VASPA (Variant Assisted SILAC Proteomic Analysis) to test the hypothesis that Drosophila male seminal fluid RNA is translated by females. We find evidence for 166 male-derived, female-translated proteins (mdFTPs) in female lower reproductive tracts at six hours postmating, many with predicted functions relevant to reproduction.

2024-06-18 | PXD041195 | Pride

Alterations in alternative RNA splicing in hepatocytes from high-fat diet fed C57BL/6 male mice

Project description:C57BL/6J male mice were fed a 60% HFD for 10 weeks, then hepatocytes were isolated, RNA extracted and subjected to RNAseq

2020-04-13 | GSE133400 | GEO

Islets RNA-seq from male C57Bl/6J on ketogenic diet

Project description:Islets RNA-seq from male C57Bl/6J on ketogenic diet

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ChIP-Seq of Gabpa in male C57Bl/6 mice liver

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Gut microbiome of Male C57Bl/6j mice fed with fructose

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Gene expression profiling in left cardiac ventricles of C57Bl/6J and C57Bl/6N male mice treated for 48 h with angiotensin II 500 ng/kg/min.

Project description:Analysis of angiotensin II effect on left ventricle at gene expression level. The hypothesis tested in the present study was that angiotensin II treatment may affect gene expression in left ventricle in a strain specific manner. Results provide important information about which genes respond to angiotensin II in C57Bl/6N male mice compared to their C57Bl/6J counterparts.

2014-12-12 | GSE53269 | GEO

ChIP-Seq of Gabpa in male C57Bl/6 mice liver

Project description:To determine genomic sites of direct GAbpα recruitment in male C57Bl/6 mice liver tissue, we performed a cistrome analysis by chromatin immunoprecipitation using a GAbpα-specific antibody followed by massive parallel DNA sequencing (ChIP-Seq).

2017-06-29 | E-MTAB-5831 | biostudies-arrayexpress

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