Mayfield algae AGP
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB33496 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR3433130.fastq.gz | Fastqsanger.gz | |||
| ERR3433140.fastq.gz | Fastqsanger.gz | |||
| ERR3433150.fastq.gz | Fastqsanger.gz | |||
| ERR3433160.fastq.gz | Fastqsanger.gz | |||
| ERR3433170.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 189 |
Similar Datasets
Project description:The metabolites derived from microalgae have been attributed with various nutritional and medicinal properties. Therefore, our study aimed to investigate the potential beneficial effects of Chlamydomonas reinhardtii (red), a type of microalgae, in individuals with type 2 diabetes mellitus (T2DM). Mice were fed on high-fat diet and injected with a low dose of streptozotocin to induce T2DM. The diabetic mice were orally treated with either 1% sodium carboxymethylcellulose or Chlamydomonas reinhardtii (red) at doses of 1, 2, or 3 g/kg BW/day for a duration of 4 weeks. The liver sections were subjected to hematoxylin and eosin staining as well as oil red staining for the detection of pathological changes and lipid deposition, respectively. Inflammatory factors in serum were quantified using ELISA kits, while commercial kits were employed to assess oxidative stress-related indicators. Gene expression in liver was analysed by RNA-seq. The results revealed that Chlamydomonas reinhardtii (red) significantly ameliorated fasting blood glucose levels, body weight, triglyceride, and low density lipoprotein cholesterin, while also enhancing oral glucose tolerance and insulin sensitivity. In pathological analysis, Chlamydomonas reinhardtii (red) significantly improved lipid deposition and hepatic tissue damage. Furthermore, Chlamydomonas reinhardtii (red) could obviously decreased the protein expression of G-6-Pase and PEPCK, and regulated the SOCS2/JAK2/STAT5 signaling pathway. Transcriptomic analysis indicated that a total of 972 significantly differentially expressed genes in diabetic mice treated with Chlamydomonas reinhardtii (red). KEGG analyses revealed that lipid and atherosclerosis, MAPK signaling pathway, B cell receptor signaling pathway, TNF signaling pathway, NOD-like receptor signaling pathway, Toll-like receptor signaling pathway, PI3K-Akt signaling pathway were involved in Chlamydomonas reinhardtii (red) modulated process. Therefore, the continuous consumption of Chlamydomonas reinhardtii (red) may have anti-T2DM effects through the inhibition of gluconeogenesis, thus offering a promising alternative for T2DM patient.
2025-01-31 | GSE287670 | GEO
Project description:Maternal stress occuring during gestation can influence the functioning of the stress and immune responses in offspring. Maternal supplementation with immunomodulatory compounds such as omega-3 polyunsaturated fatty acids may reduce inflammation associated with maternal stress, promoting offspring health and growth Sows were fed diets supplemented with microalgae or fish oil from gestation day 75 and were challenged with LPS on gestation day 112. Previously, LPS challenge in the male piglets of these sows resulted in differences in fever and cortisol responses among piglets from different maternal treatments. Therefore, the adrenal gland was chosen for microarray analysis to assess the effects of the maternal treatment on the gene expression within the hypothalamic-pituitary-adrenal axis.
2019-08-14 | GSE135782 | GEO
Project description:Nitrogen starvation is an efficient environmental pressure used to increase lipid accumulation and oil droplet formation in microalgal cells. Various studies focused on metabolic changes occurring in microalgae in nitrogen starvation conditions, but the mechanisms at the basis of these changes are not completely understood. Between microalgae, green algae, with more than 7000 species growing in a variety of habitats, have been frequently studied for energy purposes, but also as source of bioactive extracts/compounds. In this study, de novo transcriptome of the green algae Tetraselmis suecica has been performed in order to (1) deeply study its response to nitrogen starvation, (2) to look for enzymes with antioxidant capacity and for polyketide synthases (PKSs), (3) if present, to evaluate if nutrient starvation can influence their expression levels.
2018-12-01 | GSE109461 | GEO
Project description:We conducted a culture experiment by deeply submerging plants in swine wastewater in culturing Iris tectorum and co-culturing Iris tectorum and Dictyosphaerium sp., and found that the plants grew sub-normal in the plant-microalgae co-culture while the plants were dead after 21 days in the plant culture. We generated a comprehensive RNA-seq dataset from the submerged Iris tectorum leaves in both the plant culture and the plant-microalgae co-culture, aiming at providing information on the response mechanisms of the plants to waterlogging stress. Besides raw reads of the RNA-seq dataset, we used DEseq2 algorithms to detect the differently expressed genes in the plants between the different cultures. Additionally, we performed the plant disease resistance gene analysis for all the differentially expressed genes.
2018-11-03 | GSE122095 | GEO
Project description:Microalgae, particularly Chlamydomonas, produce valuable pigments like chlorophylls and carotenoids for industrial applications while demonstrating potential in bioremediation. This study employs proteomics to analyze protein expression patterns between norflurazon-resistant and non-resistant Chlamydomonas strains. The investigation aims to understand the molecular mechanisms underlying herbicide resistance, focusing on differential protein expression when exposed to norflurazon, a commonly used herbicide in weed control. This research could provide insights into both bioremediation capabilities and herbicide resistance mechanisms in microalgae.
2024-11-21 | PXD057458 | JPOST Repository
Project description:Like many microalgae unicellular green alga, Chlamydomonas reinhardtii also accumulates energy rich storage lipid and starch under nutrient-limited conditions, such as phosphorus (P) and nitrogen (N) limitation. To dissect the transcriptional regulation of lipid and starch biosynthesis in response to nutrient limitation, we have characterized the biological role of a candidate regulator: the MYB family transcription factor, PSR1 (phosphorus starvation response 1). Genetically modified (psr1 compromised) and wild-type lines were grown under P limiting and sufficient conditions and assayed at two time points by SOLiD RNA-seq.
2016-01-08 | E-MTAB-2556 | biostudies-arrayexpress
Project description:Chlamydomonas, a green algae, is known to respond to changes in light intensity by altering its protein expression. In low light conditions, Chlamydomonas increases the expression of photosynthetic genes and the proteins they encode, including the light-harvesting complexes and the reaction center proteins. This allows the algae to maximize its ability to capture light and perform photosynthesis efficiently. On the other hand, under high light conditions, Chlamydomonas reduces the expression of these photosynthetic genes to avoid photoinhibition and damage to the photosynthetic machinery. Instead, it increases the expression of stress response genes and the corresponding proteins, such as antioxidant enzymes, which protect the algae from the harmful effects of excess light. In addition, Chlamydomonas also modulates its expression of other genes and proteins, such as those involved in carbon and nitrogen metabolism, in response to changes in light intensity. This helps the algae to maintain a balance between energy production and utilization, ensuring its survival and growth under different light conditions. Overall, the ability of Chlamydomonas to modulate its protein expression in response to changes in light intensity is an important mechanism for adapting to its environment and ensuring its survival and growth.
2023-02-13 | PXD040080 | JPOST Repository
Project description:This data was generated to identify the molecular pathways responsible for nitrous oxide synthesis by the green algae Chlamydomonas reinhardtii, when supplied with nitrite under aerobic conditions (oxia). RNA samples were collected at three time points, 15 min, 3 hours, and 24 hours after the start of the experiment. The control and treatment groups were grown under the same conditions, except treatment group was supplied with 10mM nitrite at time 0. Illumina TruSeq stranded RNA libraries were synthesised from the resulting RNA before sequencing on a HiSeq2500 (125bp). The resulting sequence run generated 241,151,809 paired-end 125bp reads, of which 200,946,839 remained following quality filtering. The short data was mapped to the published genome and read counts were generated with HT-Seq count with the default settings. The raw read count data was analysed by DESeq2 in order to identify genes differentially expressed during nitrous oxide production.
2017-07-01 | GSE90609 | GEO
Project description:Metatranscriptome of microalgae axenic culture microbial communities from LLNL, Livermore, California, USA - f15_d2_W_S24
| PRJNA1341105 | ENA
Project description:Metatranscriptome of microalgae axenic culture microbial communities from LLNL, Livermore, California, USA - f28_d5_W_S43
| PRJNA1341262 | ENA