Project description:Formalin induces inter- and intra-molecular crosslinks within exposed cells. This cross-linking can be exploited to characterise chromatin state as in the MNase (micrococcal nuclease) assays. Our team aims to optimise these assays for application in museum preserved formalin-exposed specimens. To do so, we applied an optimised MNase assay to fresh-frozen and archival eastern water dragon specimens, as old as 1905. We found that heavy formalin fixation modulates rather than eliminates signatures of differential chromatin accessibility and that these chromatin profiles are sex-specific and environmental condition dependent.
Project description:This dataset contains TMT-10plex quantitative proteomics of serum isolated from monogamous deer mice (Peromyscus). The study investigates the systemic physiological impact of social interactions, specifically comparing pair-bonded animals to those that have experienced bond disruption. The circulating serum proteome was analyzed to identify factors that mediate peripheral tissue responses to social history. This serum data supports findings regarding the impact of social bonds on the lung transcriptome (Naderi et al., 2023, BMC Genomics) and systemic transcriptomic stochasticity/gene canalization in cultured cells.
Project description:Whole transcriptome RNA sequencing in primary fibroblasts of outbred Peromyscus maniculatus was generated to explore the degree of coordination in gene expression.