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Mapping of MLE and MSL2 using iCLIP

ABSTRACT: Mapping of MLE and MSL2 using iCLIP

PROVIDER: PRJEB3406 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR297217_1.fastq.gz	Fastqsanger.gz
	ERR297217_2.fastq.gz	Fastqsanger.gz
	ERR297218_1.fastq.gz	Fastqsanger.gz
	ERR297218_2.fastq.gz	Fastqsanger.gz
	ERR297219_1.fastq.gz	Fastqsanger.gz

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1 - 5 of 12

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Mapping of MLE and MSL2 using iCLIP

Project description:Dosage compensation in Drosophila is an epigenetic phenomenon utilizing proteins and long non-coding RNAs (lncRNAs) for transcriptional up-regulation of the entire X-chromosome. Here, UV cross-linking followed by deep sequencing (iCLIP) show that two enzymes in the Male-Specific Lethal complex, MLE RNA helicase and MSL2 ubiquitin ligase, bind evolutionarily conserved domains containing tandem stem-loops in roX1 and roX2 RNAs in vivo.

2013-07-18 | E-MTAB-1669 | biostudies-arrayexpress

ChIP-Seq profiles of MSL1, MSL2, MSl3, MOF, MLE, H4K16ac and RNA Polymerase II phosphorlyated on Serine 5 in Drosophila S2 cells

Project description:ChIP-Seq profiles of MSL1, MSL2, MSl3, MOF, MLE, H4K16ac and RNA Polymerase II phosphorlyated on Serine 5 in Drosophila S2 cells MSL1, MSL2, MSL3, MOF, MLE, H4K16ac and RNA Polymerase II phosphorlyated on Serine 5 ChIP in Drosophila S2 cells. 1-3 biological replicates per experiment. Performed in single-read and paired-end read mode.

2012-12-11 | E-GEOD-37864 | biostudies-arrayexpress

ChIP-Seq profiles of MSL1, MSL2, MSl3, MOF, MLE, H4K16ac and RNA Polymerase II phosphorlyated on Serine 5 in Drosophila S2 cells

Project description:ChIP-Seq profiles of MSL1, MSL2, MSl3, MOF, MLE, H4K16ac and RNA Polymerase II phosphorlyated on Serine 5 in Drosophila S2 cells

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The interaction of MLE with CLAMP zinc finger domains is important for dosage compensation in Drosophila melanogaster

Project description:The Drosophila male-specific lethal (MSL) complex binds to the male X chromosome to activate transcription, and consists of five proteins, MSL1, MSL2, MSL3, MOF, MLE, and two roX RNAs. The MLE helicase remodels the roX lncRNAs, enabling the lncRNA-mediated assembly of the Drosophila dosage compensation complex. MSL2 is expressed only in males and interacts with the N-terminal zinc-finger of the transcription factor CLAMP that is important for specific recruitment of the MSL complex on the male X chromosome. Here we found that the unstructured C-terminal region of MLE interacts with 6-7 zinc-finger domains of CLAMP. In vitro 4-5 zinc fingers are critical for specific DNA-binding of CLAMP with GA-repeats, which constitute the core motif at the high affinity binding sites for MSL proteins. Deletion of the Clamp Binding Domain (CBD) in MLE results in decreasing of MSL proteins association with male X chromosome and increasing of male lethality. These results suggest that interactions of unstructured regions in MSL2 and MLE with CLAMP zinc finger domains are important for the specific recruitment of the MSL complex on the male X chromosome.

2024-03-20 | GSE239354 | GEO

Transcriptome-wide mapping of hnRNP C and U2AF65 using iCLIP

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iCLIP transcriptome-wide mapping of UNR (CSDE1) binding sites

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MLE ChIP-seq

Project description:Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) was carried out on wild-type Schneider (S2) cells using specific MLE antibodies to identify binding sites for MLE in the Drosophila genome

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Transcriptome-wide mapping of hnRNP C and U2AF65 using iCLIP

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2013-01-31 | E-MTAB-1371 | biostudies-arrayexpress

MLE ChIP-seq

Project description:MLE ChIP-seq

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MLE HITS-CLIP in Drosophila S2 cells

Project description:HITS-CLIP was carried out on wild-type Schneider (S2) cells using specific MLE antibodies to identify binding sites for MLE in the Drosophila transcriptome

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