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RNA-seq of mouse subcutaneous SVF cells before and after differentiation with a brown fat differentiation cocktail

ABSTRACT: RNA-seq of mouse subcutaneous SVF cells before and after differentiation with a brown fat differentiation cocktail

PROVIDER: PRJEB34685 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3568690.fastq.gz	Fastqsanger.gz
	ERR3568700.fastq.gz	Fastqsanger.gz
	ERR3568710.fastq.gz	Fastqsanger.gz
	ERR3568720_1.fastq.gz	Fastqsanger.gz
	ERR3568720_2.fastq.gz	Fastqsanger.gz

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RNA-seq of mouse subcutaneous SVF cells before and after differentiation with a brown fat differentiation cocktail

Project description:White fat browning is a highly variable genetic trait in mice (Guerra et al., 1998). To gain an overview of strain variations in browning capacities, we performed transcriptome analysis of white fat browning in (1) 5 inbred mouse strains (C57BL/6J, 129S6sv/ev, A/J, AKR/J, and SWR/J) with distinct browning propensities in WAT, and (2) F1 hybrids derived from a high (129S6sv/ev) and low browning strain (C57BL/6J) cross. White fat browning is a highly variable genetic trait in mice (Guerra et al., 1998). To gain an overview of strain variations in browning capacities, we performed transcriptome analysis of white fat browning in three genetic models (Figure 1A), including (1) 5 inbred mouse strains (C57BL/6J, 129S6sv/ev, A/J, AKR/J, and SWR/J) with distinct browning propensities in WAT, (2) F1 hybrids derived from a high (129S6sv/ev) and low browning strain (C57BL/6J) cross.

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Gene expression data of brown-like adipocytes from Lgr4 homozygous mutant SVF

Project description:Obesity has become a global health problem. Brown adipose tissue (BAT), specialized for energy expenditure through thermogenesis, potently counteracts obesity. Recently, BAT is also identified in human adults. We found that Lgr4 homozygous mutant (Lgr4m/m) mice display reduced adiposity and exhibit brown-like adipocytes in their WAT depots with higher expression of uncoupling protein 1 (Ucp1). Furthermore, Lgr4 ablation potentiates brown adipocyte differentiation from stromal vascular fraction (SVF) of epididymal WAT (eWAT) in vitro. We used microarrays to emxamine the gene expression profiles of the brown-like adipocytes differentiated from SVF of wild-type and Lgr4 mutant mice. We identified distinct gene expression profiles of these two groups. To demonstrate Lgr4 ablation can potentiate the differentiation of SVF from eWAT toward brown-like adipocytes in vitro, we isolated SVF from epididymal white adipose tissue (eWAT) of wild-type (WT) and Lgr4 mutant mice. We then plated SVF cells in 12-well plate, and differentiated them to brown adipocytes, followed by RNA extraction and hybridization with Affymetrix microarrays.

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Gene expression data of brown-like adipocytes from Lgr4 homozygous mutant SVF

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Project description:3' RNA-seq of differentiating (brown) mouse subcutaneous SVF cells upon knockdown of Zfp521, Fhl1, or Mxd1

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Characterization of Mmp3 positive and negative cells from SVF of subcutaneous adipose tissue

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