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Single cell RNA sequencing of DDX4 antibody positive and negative ovarian tissue cells cultured under oogonial stem cell conditions

ABSTRACT: Single cell RNA sequencing of DDX4 antibody positive and negative ovarian tissue cells cultured under oogonial stem cell conditions

PROVIDER: PRJEB34730 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3577250.fastq.gz	Fastqsanger.gz
	ERR3577260.fastq.gz	Fastqsanger.gz
	ERR3577270.fastq.gz	Fastqsanger.gz
	ERR3577280.fastq.gz	Fastqsanger.gz
	ERR3577251.fastq.gz	Fastqsanger.gz

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Single cell RNA sequencing of DDX4 antibody positive and negative ovarian tissue cells cultured under oogonial stem cell conditions

Project description:Human ovarian tissue cells positive for DDX4 on the surface were claimed to be of oogonial stem cell nature. In order to study DDX4 antibody positive putative oogonial stem cells, ovarian tissue cells were sorted into DDX4 antibody positive and negative populations and single cell RNA-seq was performed using Smart-seq2.

2020-01-30 | E-MTAB-8403 | biostudies-arrayexpress

Germline-specific RNA helicase DDX4 forms cytoplasmic granules in cancer cells and promotes tumor growth

Project description:Cancer cells undergo major epigenetic alterations and transcriptome changes, including ectopic expression of many tissue- and cell type-specific genes. Here we show that the germline-specific RNA helicase DDX4 forms germ granule-like cytoplasmic ribonucleoprotein (RNP) granules in various human and mouse tumors, but not in cultured cancer cells. The DDX4-compexes in tumors contain RNA-binding proteins and splicing regulators, including many known germ granule components. The deletion of DDX4 in cancer cells induces transcriptome imbalance and affects the alternative splicing landscape of a large number of genes with reported functions in cancer growth and invasiveness, leading to their compromised capability to form xenograft tumors. Importantly, high expression of DDX4-positive granules is associated with poor survival in patients with squamous cell carcinoma of the head and neck, as well as with the histological grade of prostate cancer. Taken together, these results show that the formation of germ granule-resembling DDX4 granules have prognostic value, and they are involved in transcriptome control in cancer cells to promote malignant properties.

2024-06-10 | PXD044856 | Pride

Expression data from cultured human ovarian carcinoma cell lines

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Expression data from cultured human ovarian carcinoma cell lines

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Effect of SASP on human Ovcar8 ovarian cancer cell line cultured in ultra-low attachment conditions

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Germline-specific RNA helicase DDX4 forms cytoplasmic granules in cancer cells and promotes tumor growth

Project description:Cancer cells undergo major epigenetic alterations and transcriptomic changes, including ectopic expression of many tissue- and cell type-specific genes. Here we show that the germline-specific RNA helicase DDX4 (DEAD-box helicase 4) forms germ granule-like cytoplasmic ribonucleoprotein (RNP) granules in various human tumors, but not in cultured cancer cells. These cancerous DDX4-complexes contain RNA-binding proteins and splicing regulators, including many known germ granule components. The deletion of DDX4 in cancer cells induces transcriptomic changes and affects the alternative splicing landscape of a large number of genes involved in cancer growth and invasiveness, leading to compromised capability of DDX4-null cancer cells to form xenograft tumors in immunocompromised mice. Importantly, occurrence of DDX4-granules is associated with poor survival in patients with head and neck squamous cell carcinoma, and higher histological grade of prostate cancer. Taken together, these results show that the germ granule-resembling cancerous DDX4-granules control gene expression and promote malignant and invasive properties of cancer cells.

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Gene expression in NIH:OVCAR-3 cells cultured in different cell density conditions

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Single cell profilng of hCS derived from hiPSC cultured in feeder-free conditions

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Transcriptomes of high-grade serous ovarian cancer cell lines cultured in monolayer and in spheroids.

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Gene expression in cultured bovine ovarian granulosa

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2014-05-20 | GSE42535 | GEO

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