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Aquatic environmental monitoring using integrated Omics approaches

ABSTRACT: Quantitative transcriptomics in evaluating ovarian developmental effects in Atlantic cod (Gadus morhua) caged at a capped waste disposal site

PROVIDER: PRJEB34872 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR3697640_1.fastq.gz	Fastqsanger.gz
	ERR3697640_2.fastq.gz	Fastqsanger.gz
	ERR3697650_1.fastq.gz	Fastqsanger.gz
	ERR3697650_2.fastq.gz	Fastqsanger.gz
	ERR3697641_1.fastq.gz	Fastqsanger.gz

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Atlantic Cod Early Development

Project description:Gene expression profiling of Atlantic cod (Gadus morhua) embryogenesis

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Microarray analysis of gene expression of Atlantic cod from different Baltic Sea regions

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Project description:The Atlantic cod (Gadus morhua L.) is one of the most important species in the Baltic Sea with high ecological and economical value. To explore the differences in adaptation to salinity between Baltic cod subpopulation: western (Kiel Bight) and eastern (Gdańsk Bay) samples were analyzed through genome-wide oligonucleotide microarray.

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Project description:Aquaculture is one of the fastest growing food production sectors in the world and further expansion is expected throughout the 21st century. However, climate change is threatening the development of the sector and action is needed to prepare the industry for the coming challenges. Using downscaled temperature projections based on the Intergovernmental Panel on Climate Change (IPCC) climate projection (Shared Socioeconomic Pathway, SSP2-4.5), we analysed potential future temperatures at a selected Atlantic cod (Gadus morhua L.) farm site in Northern Norway. Results showed that the farming area may experience increased temperatures the next 10–15 years, including more days with temperatures above 17°C. Based on the predicted future conditions, we designed a study with Atlantic cod (Gadus morhua L.) to evaluate effects from high temperature alone and in combination with Fransicella noatunensis infection. Fish were kept at 12°C and 17°C for eight weeks and samples of skin and spleen collected at different timepoints were analysed with transcriptomics, histology, scanning electron microscopy and immunohistochemistry.

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fGadMor1

Project description:Gadus morhua (Atlantic cod)

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The study of Atlantic cod (Gadus morhua) spleen global gene expression response to intraperitoneal injection with formalin-killed atypical Aeromonas salmonicida.

Project description:This study was performed to validate the newly developed CGP Atlantic cod 20K oligonucleotide microarray. Atlantic cod (Gadus morhua) received an intraperitoneal injection of either formalin-killed, atypical Aeromonas salmonicida (Asal) or PBS and transcriptional profiles of spleen tissues from Asal-injected fish were compared to those from pre-injection control fish and PBS-injected control fish. Gene expression profiles resulting from this study were compared to those from suppression subtractive hybridization library studies, that were previously performed on the same samples, and to literature. Gene expression patterns of single genes were confirmed by QPCR analysis. This study has shown that the newly developed CGP Atlantic cod 20K oligo microarray platform is a valuable tool for cod genomic research.

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Effects on the lipid metabolism of Atlantic cod (Gadus morhua) after in vivo exposure with Ppar agonists

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2022-09-20 | PXD031739 | Pride

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| PRJNA1084037 | ENA