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RNA-seq of phage VPE25 treated Enterococcus faecalis OG1RF relative to untreated controls

ABSTRACT: RNA-seq of phage VPE25 treated Enterococcus faecalis OG1RF relative to untreated controls

PROVIDER: PRJEB35542 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR3680690_1.fastq.gz	Fastqsanger.gz
	ERR3680690_2.fastq.gz	Fastqsanger.gz
	ERR3680700_1.fastq.gz	Fastqsanger.gz
	ERR3680700_2.fastq.gz	Fastqsanger.gz
	ERR3680691_1.fastq.gz	Fastqsanger.gz

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Proteomics of phage VPE25 treated Enterococcus faecalis OG1RF relative to untreated controls

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Genome-wide Analysis of Enterococcus faecalis OG1RF(pCF10) Gene Expression During Infection

Project description:Changes in Enterococcus faecalis OG1RF(pCF10) gene expression at 4 hours post-infection in a rabbit model of subdermal abscess formation were studied using RNA-seq analysis.

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Project description:Transcriptional profiling to investigate the effect of drug treatment on the E. faecalis cells. For microarray analysis, E. faecalis OG1RF was grown in FMC medium supplemented with 10 mM glucose to an optical density at 600 nm (OD600) of 0.3 and the cultures were divided in 7 aliquots. One aliquot was collected by centrifugation and immediately frozen (untreated control cells). The other aliquots were treated for 30 or 60 min with 1.25 X the minimum inhibitory concentration (MIC) of one of the following CW inhibitors: ampicillin (20 μg ml-1), bacitracin (80 μg ml-1), or cephalothin (80 μg ml-1). After an exposure time of 30 or 60 minutes, each of these cultures was also centrifuged and the pellets frozen. RNA was then isolated from each pellet for microarray analysis. This process was repeated 3 additional times, for a total of four replicates of each condition.

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