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Time-course transcriptome analysis of mature nodules in Medicago truncatula grown in split-root under different nitrogen (N) supply

ABSTRACT: Time-course transcriptome analysis of mature nodules in Medicago truncatula grown in split-root under different nitrogen (N) supply

PROVIDER: PRJEB35811 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3762980.fastq.gz	Fastqsanger.gz
	ERR3762990.fastq.gz	Fastqsanger.gz
	ERR3762981.fastq.gz	Fastqsanger.gz
	ERR3762991.fastq.gz	Fastqsanger.gz
	ERR3762982.fastq.gz	Fastqsanger.gz

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Time-course transcriptome analysis of mature nodules in Medicago truncatula grown in split-root under different nitrogen (N) supply

Project description:The experiment aim is the identification of the transcriptional response of mature nodules of Medicago truncatula inoculated with the strain MD4 of Sinorhizobium medicae in response to different nitrogen (N) supply (N-satiety and N-deficient conditions). We performed a time-course (1 and 3 days treatment) split-root experiment to identify plant systemic responses: the root systems of nodulated symbiotic plants were divided into two compartments, one of them treated and the other kept on control conditions. Nodules were isolated from the non-treated side of the split root and total RNA isolated for RNAseq analysis.

2020-09-30 | E-MTAB-8597 | biostudies-arrayexpress

Time-course transcriptome analysis of Sinorhizobium medicae (MD4 strain) in mature nodules of Medicago truncatula grown in split-root under different nitrogen (N) supply

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Time-course transcriptome analysis of Sinorhizobium medicae (MD4 strain) in mature nodules of Medicago truncatula grown in split-root under different nitrogen (N) supply

Project description:Time-course transcriptome analysis of Sinorhizobium medicae (MD4 strain) in mature nodules of Medicago truncatula grown in split-root under different nitrogen (N) supply

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Processing of NODULE INCEPTION controls transition to nitrogen fixation in root nodules

Project description:To understand the role of Medicago truncatula NODULE INCEPTION (MtNIN) in nodule development, we analyzed the transcriptome of roots ectopically overexpressing NIN, nodules of weak mutant allele nin-16, and nodules of dnf1-2 mutant.

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Response of the mature nodules and denodulated roots of the Medicago truncatula/sinorhizobium medicae md4 symbiotic association to systemic signaling of plant N deficit

Project description:The experiment aims to identify the response of mature nodules and denodulated roots of Medicago truncatula /Sinorhizobium medicae md4 to the systemic signaling of plant N deficit. The root systems of nodulated symbiotic plants were divided into two compartments. The N source of N2 fixing plants was exclusively the air. To reduce the plant nitrogen (N) supply (N-deficient conditions), we flushed one half root system with Ar/O2 (80/20 v/v) while the other half root system was kept on control conditions (Air). To characterize the systemic responses of the transcriptome to plant N deficit , nodules and roots of the non-treated half root system were harvested and total RNA isolated for RNAseq analysis. We performed a time-course (6 hours, 1-, 3- and 5-days treatment).

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Project description:We have used deep sequencing of small RNAs from nodules and root apexes of the model legume Medicago truncatula, to identify 113 novel candidate miRNAs. These miRNAs (legume or Mt-specific) are encoded by 278 putative hairpin precursors in the M. truncatula genome. Several miRNAs are differentially expressed in nodules and root tips and large variety of targets could be predicted for these genes. Specific miRNA isoforms showed contrasting expression patterns in these tissues Keywords: Transcriptome analysis

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