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Establishment of an in vitro human blood-brain barrier model derived from induced pluripotent stem cells

ABSTRACT: Transcriptome sequencing of human induced pluripotent stem cell-derived brain endothelial cells. Overall design: Examined one time point (Day 10 of differentiation) from two different rounds of iPSC BMECs differentiation. Sample name: Cl1 (Clone 1) Total samples: 2 (two different rounds of differentiation)

PROVIDER: PRJEB35916 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3829230.fastq.gz	Fastqsanger.gz
	ERR3829650.fastq.gz	Fastqsanger.gz
	ERR3829231.fastq.gz	Fastqsanger.gz
	ERR3829651.fastq.gz	Fastqsanger.gz
	ERR3829232.fastq.gz	Fastqsanger.gz

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MicroRNA-449a Enhances Radiosensitivity in Lung Adenocarcinoma Cells

Project description:Twenty-five miRNAs were identified as having differential expression post-irradiation in CL1-0 or CL1-5 cells. Among these miRNAs, miR-449a, which was down-regulated in CL1-0 cells at 24 h after irradiation, was chosen for further investigation. Overexpression of miR-449a in CL1-0 cells effectively increased irradiation-induced DNA damage and apoptosis, altered the cell cycle distribution and eventually led to sensitization of CL1-0 to irradiation. MiR-449a might be a novel radiosensitizer for clinical applications. Two lung adenocarcinoma cell lines (CL1-0 and CL1-5) with different metastatic ability and radiosensitivity were used. In order to understand the regulatory mechanisms of differential radiosensitivity in these isogenic tumor cells, both CL1-0 and CL1-5 were treated with 10 Gy radiation, and were harvested respectively at 0, 1, 4, and 24 h after radiation exposure. The changes in expression of miRNA upon irradiation were examined using Illumina Human microRNA BeadChips.

2012-12-30 | E-GEOD-40602 | biostudies-arrayexpress

Temporal changes of floral color and expression of carotenoids-related genes in flower petal of Osmanthus fragrans var. semperflorens : using de novo transcriptome sequencing

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Establishment of a bovine rumen epithelial cell line

Project description:The goal of this study was to characterize a single-cell clone derived from bovine rumen epithelium. Analyses including RNA-seq demonstrated that this clone was derived from a rumen epithelial cell. This clone is named BREC1 in the manuscript.

2021-10-27 | GSE181659 | GEO

Testing for gene expression differences between chromosomally stable and unstable isogenic clones

Project description:An irradiated but chromosomally stable CHO clone RT210B was used a reference to find differences in gene expression compared to radiation-induced chromosomally unstable isogenic clones. Equal quantities of two unstable different unstable clones were mixed prior to RNA extraction. Each mixture was tested twice versus the stable reference clone. Two different two clone mixtures were tested against the same chromosomally stable clone Keywords: repeat sample

2004-08-01 | GSE1626 | GEO

Transcription profiling of human hematopoietic stem cells vs. differentiated progeny

Project description:Hematopoietic stem cells (HSC) continuously regenerate a complete hematologic and immune system. Very few genes that regulate this process have yet been identified. In order to identify factors governing differentiation, we have compared the transcriptome of highly purified HSC with their differentiated progeny, including erythrocytes, granulocytes, monocytes, NK cells, activated and naÃ¯ve T-cells, and B-cells. Chromosomal analysis revealed that HSC were more transcriptionally active than other cell types across most chromosomes. Each lineage expressed ~100 to 400 genes uniquely, including many previously uncharacterized genes. Overexpression of two fingerprint genes resulted in a significant bias in differentiation indicating a role in cell fate determination, demonstrating the utility of these data for modulation of specific cell types. Experiment Overall Design: Goal - Expression profiling of hematopoietic cells Experiment Overall Design: Description - Biological replicates of 10 hematopoietic cell types from female C57Bl/6 mice were purified for microarray analysis Experiment Overall Design: Experimental Factors: wild-type female C57Bl/6 CD45.1 Mus musculus Experiment Overall Design: Experimental Design: 25,000- 50,000 cells were FACS sorted, mRNA was purified by RNAqueous (Ambion), DNAse treated with DNAse1, phenol:chloroform:isoamyl alcohol extracted, and amplified through two rounds of in vitro transcription using MessageAmp (Ambion). Second round was biotinylated with bio-CTP and bio-UTP. Experiment Overall Design: Microarrays: Oligonucleotide, Affymetrix, Moe430 v 2.0 Experiment Overall Design: QC: Biological replicates, correlation coefficient > .95; scale factor <10; 5'/3' ratio < 20

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Transcription profiling of mouse multipotent adult progenitor cells

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2008-06-15 | E-GEOD-6933 | biostudies-arrayexpress

Expression data from CL1-0 and CL1-5 lung cancer cell line.

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Transcription profiling of rat glomeruli: conventionally-isolated and laser capture microdissected glomeruli

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2008-06-17 | E-GEOD-8998 | biostudies-arrayexpress

Transcription profiling of human pre-invasive and invasive cervical squamous cell carcinomas and normal cervical epithelia

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