Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RiboSeq of murine 17Cl-1 cells infected at MOI 10 with MHV-A59, compared to mock-infected controls, harvested at 5 and 8 hpi, plus a control tunicamycin-treated sample.

ABSTRACT: RiboSeq of murine 17Cl-1 cells infected at MOI 10 with MHV-A59, compared to mock-infected controls, harvested at 5 and 8 hpi, plus a control tunicamycin-treated sample.

PROVIDER: PRJEB36148 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR3804460.fastq.gz	Fastqsanger.gz
	ERR3804461.fastq.gz	Fastqsanger.gz
	ERR3804462.fastq.gz	Fastqsanger.gz
	ERR3804463.fastq.gz	Fastqsanger.gz
	ERR3804464.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 7

Similar Datasets

RNASeq of murine 17Cl-1 cells infected at MOI 10 with MHV-A59, compared to mock-infected controls, harvested at 5 and 8 hpi.

Project description:Murine 17Cl-1 cells were infected with the model Betacoronavirus mouse hepatitis virus (MHV) strain A59 to observe changes in the host transcriptome in infected cells compared to mock-infected cells. Samples were harvested at 5 and 8 hpi by flash-freezing, without cycloheximide pre-treatment. Ribosomal RNA was removed using Illumina's RiboZero kit, and RNA was hydrolysed then gel purified to select fragments 25-35nt long. Fragments were cloned into adapters using the TruSeq small RNA adapter kit and sequenced on Illumina NextSeq, then differential gene expression analysis was carried out on the data deposited data.

2021-05-18 | E-MTAB-8651 | biostudies-arrayexpress

LC-MSMS of Mice BMDC infected by MHV-A59

Project description:We conducted a comprehensive analysis of the proteome of mouse bone marrow dendritic cells (BMDCs) infected with MHV-A59. We characterized the global gene changes at the protein levels following viral infection, identifying ten genes involved in various anti-MHV biological processes.

2025-06-06 | PXD061826 | Pride

RiboSeq of murine 17Cl-1 cells infected at MOI 10 with MHV-A59, compared to mock-infected controls, harvested at 5 and 8 hpi, plus a control tunicamycin-treated sample.

Project description:Murine 17Cl-1 cells were infected with the model Betacoronavirus mouse hepatitis virus (MHV) strain A59 and subjected to ribosome profiling to observe changes in the host translatome in infected cells compared to mock-infected cells. Samples were harvested at 5 and 8 hpi by flash-freezing, without cycloheximide pre-treatment. RNase I treatment was carried out, following which ribosomes and enclosed RNA were isolated by centrifugation through a sucrose cushion. RNA was extracted, ribosomal RNA was removed using Illumina's RiboZero kit, and remaining RNA was gel purified to select fragments 25-35nt long. Fragments were cloned into adapters using the TruSeq small RNA adapter kit and sequenced on Illumina NextSeq, then differential translation efficiency analysis was carried out on the data deposited data. One uninfected sample was treated with 2ug/mL tunicamycin for 6 hours before harvesting as a positive control for unfolded protein response activation.

2021-05-18 | E-MTAB-8650 | biostudies-arrayexpress

Bulk RNA-seq of MHV-A59 infection in primary murine macrophages

Project description:Primary mouse peritoneal macrophages and bone marrow derived macrophages were isolated and infected with MHV-A59.

2021-10-20 | GSE185800 | GEO

Gene expression differences between wild-type and ZUP1 knock-out mice in liver infected with MHV-A59

Project description:To investigate the transcriptome differences between wild-type and ZUP1 knock-out mice in liver when they were infected with MHV, we employed MHV-A59, a hepatic and neuronal tropic murine coronavirus, to infect wild-type and Zup1 knockout mice and colleted liver tissues after MHV infection to perform RNA- sequencing.

2025-01-16 | GSE286528 | GEO

RNASeq of murine 17Cl-1 cells infected at MOI 10 with MHV-A59, compared to mock-infected controls, harvested at 5 and 8 hpi.

Project description:RNASeq of murine 17Cl-1 cells infected at MOI 10 with MHV-A59, compared to mock-infected controls, harvested at 5 and 8 hpi.

| PRJEB36149 | ENA

dsRNA IP of Coronavirus Infected IFNAR-/- BMDMs

Project description:RNA-sequencing data of mock, wild-type, and EndoUmut MHV A59 infected IFNAR-/- bone-marrow derived macrophages. RNA was isolated at 6 hours post infection and immunoprecipitated with anti-dsRNA antibody to determine which RNA are forming a dsRNA epitope during viral infection. RNAs were mapped to C57Bl/6 Mouse genome or MHV-A59 genome (Genbank accession # AY910861).

2020-02-07 | GSE144886 | GEO

Bulk RNA-seq of MHV-A59 infection in primary murine macrophages

Project description:Bulk RNA-seq of MHV-A59 infection in primary murine macrophages

| PRJNA770889 | ENA

Gene expression differences between wild-type and ZUP1 knock-out mice in liver infected with MHV-A59

Project description:Gene expression differences between wild-type and ZUP1 knock-out mice in liver infected with MHV-A59

| PRJNA1209862 | ENA

Transcription profiling of mouse fibroblasts infected with murine hepatitis virus using two array platforms to compare the potential for interference from viral RNA

Project description:Infection of cells with murine hepatitis virus strain A59 (MHV-A59) results in massive amounts of viral RNA within infected cells. When applying transcriptional profiling by means of microarray analysis, this could potentially cause problems since high amounts of viral RNA could in theory interfere with the analysis. Since coronavirus RNAs are polyadenylated, the viral RNAs are also amplified by oligo(dT) primers (a standard procedure when processing RNA samples for array hybridization). In this experiment we compared two different array platforms for the potential interference of viral RNA, using MHV-A59 infection of LR7 (murine fibroblasts) as a model.

2008-05-21 | E-MEXP-1373 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data