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Propionic acid promotes the virulent phenotype of Crohn’s disease-associated adherent-invasive Escherichia coli

ABSTRACT: Propionic acid promotes the virulent phenotype of Crohn’s disease-associated adherent-invasive Escherichia coli

PROVIDER: PRJEB36206 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3813720_1.fastq.gz	Fastqsanger.gz
	ERR3813720_2.fastq.gz	Fastqsanger.gz
	ERR3813740.fastq.gz	Fastqsanger.gz
	ERR3813741.fastq.gz	Fastqsanger.gz
	ERR3813712_1.fastq.gz	Fastqsanger.gz

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Propionic Acid Promotes the Virulent Phenotype of Crohn's Disease-Associated Adherent-Invasive Escherichia coli.

Project description:Propionic acid (PA) is a bacterium-derived intestinal antimicrobial and immune modulator used widely in food production and agriculture. Passage of Crohn's disease-associated adherent-invasive Escherichia coli (AIEC) through a murine model, in which intestinal PA levels are increased to mimic the human intestine, leads to the recovery of AIEC with significantly increased virulence. Similar phenotypic changes are observed outside the murine model when AIEC is grown in culture with PA as the sole carbon source; such PA exposure also results in AIEC that persists at 20-fold higher levels in vivo. RNA sequencing identifies an upregulation of genes involved in biofilm formation, stress response, metabolism, membrane integrity, and alternative carbon source utilization. PA exposure also increases virulence in a number of E. coli isolates from Crohn's disease patients. Removal of PA is sufficient to reverse these phenotypic changes. Our data indicate that exposure to PA results in AIEC resistance and increased virulence in its presence.

| S-EPMC7034058 | biostudies-literature

Crohn’s Disease Differentially Affects Region Specific Composition and Aerotolerance Profiles of Mucosally-Adherent Bacteria

Project description:BACKGROUND: The intestinal microbiota play a key role in the onset, progression, and recurrence of Crohn’s disease (CD). Most microbiome studies assay fecal material, which does not provide region-specific information on mucosally adherent bacteria that directly interact with host systems. Changes in luminal oxygen has been proposed as a contributor to CD dybiosis. METHODS: 16S rRNA data was generated using colonic and ileal mucosal from patients with CD and without inflammatory bowel diseases (nonIBD). We developed profiles reflecting bacterial abundance within defined aerotolerance categories. Bacterial diversity, composition, and aerotolerance profiles were compared across intestinal regions and disease phenotypes. RESULTS: Bacterial diversity decreased in CD in both ileum and colon. Aerotolerance profiles significantly differed between intestinal segments in nonIBD, though both were dominated by obligate anaerobes, as expected. In CD, high relative levels of obligate anaerobes were maintained in the colon and increased in the ileum. Relative abundance of similar and distinct taxa were altered in colon and ileum. Notably, several obligate anaerobes, such as Bacteroides fragilis, dramatically increased in CD in one or both intestinal segments, though specific increasing taxa varied across patients. Increased abundance of taxa from the Proteobacteria phylum was found only in the ileum. Bacterial diversity was significantly reduced in resected pre-operative tissues of patients that developed disease recurrence across two independent cohorts, with common lower abundance of bacteria from the Bacteroides, Streptococcus, and Blautia genera. CONCLUSIONS: Mucosally adherent bacteria in colon and ileum show distinct alterations in CD that provide additional insights not revealed in fecal material.

2020-04-15 | GSE147600 | GEO

Crohn’s Disease Differentially Affects Region Specific Composition and Aerotolerance Profiles of Mucosally-Adherent Bacteria

Project description:Crohn’s Disease Differentially Affects Region Specific Composition and Aerotolerance Profiles of Mucosally-Adherent Bacteria

| PRJNA615672 | ENA

Gut-derived indole propionic acid alleviates liver fibrosis by modulating pro-fibrogenic macrophage phenotype and biological function

Project description:Recently, gut metabolites have been recognized to play significant roles in liver diseases via the gut-liver axis. In this study, we investigate the regulatory effects of the tryptophan metabolite, indole-3-propionic acid (IPA), on immune cells during liver fibrogenesis using single-cell RNA sequencing (scRNA-seq).

2024-09-20 | GSE277148 | GEO

Transcriptional profiing of cultured adherent and non-adherent monocytes

Project description:Transcriptional profiing of cultured adherent and non-adherent monocytes

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RELA haploinsufficiency manifesting as an atypical phenotype of Crohn’s disease

Project description:Mutations in RELA, a key component of NF-κB signaling, are associated with dysregulated immune responses and inflammatory disorders. While immunodeficiency phenotypes associated with RELA haploinsufficiency have been reported, gastrointestinal manifestations remain poorly described. We aimed to characterize the clinical, genomic, and immunological features of a patient presenting with an atypical Crohn’s-like phenotype driven by RELA haploinsufficiency. We report a case of RELA haploinsufficiency in a 17-year-old male presenting with a Crohn’s-like phenotype, including pan-enteric inflammation, perianal disease, chronic mucocutaneous candidiasis, and lymphopenia. Whole exome sequencing revealed a heterozygous RELA missense variant (p.V196A), which might impaired RelA protein stability and function. Functional assays showed reduced NF-κB activity and protein expression. Immune profiling by CyTOF and scRNA-seq demonstrated Treg deficiency, high IFN-γ levels, a strong interferon signature, and aberrant activation of MAIT and cytotoxic CD4+ T cells with upregulation of IL23R and ADAM12.

2025-05-28 | GSE298194 | GEO

Transcriptional profiing of cultured adherent and non-adherent monocytes

Project description:To determine the effect of adherence on the metabolic and functional response of human monocytes. Monocytes were stimulated with lipopolysaccharide (LPS) under non-adherent and adherent conditions. To determine the role of glycolysis in LPS-induced immune responses, this pathway was inhibited by glucose deprivation or the glucose analog 2-deoxy-D-glucose (2DG).

2020-12-08 | GSE161839 | GEO

Investigating the Mechanism of Adherent-Invasive E. coli Induced Mitochondrial Fission in the Intestinal Epithelium (Exp. 2)

Project description:RNA-seq was performed to interogate the transcriptional changes in response to infection with Adherent Invasive E. coli and a control, non-invasive E. coli

2020-07-10 | GSE154122 | GEO

Investigating the Mechanism of Adherent-Invasive E. coli Induced Mitochondrial Fission in the Intestinal Epithelium (Exp. 1)

Project description:RNA-seq was performed to interogate the transcriptional changes in response to infection with Adherent Invasive E. coli and a control, non-invasive E. coli

2020-07-10 | GSE154121 | GEO

Expression data of Mesenchymal Stroma Cells (MSC)-adherent and non-adherent, human cord blood-derived, hematopoietic CD34 cells

Project description:MSC-adherent hematopoietic stem and progenotir cells (HSPC) express adhesion-associated genes at higher levels than non-adherent cells while cell-cycle and differentiation-associated genes are not significantly changed between the two cell populations. We used microarray to confirm identity of MSC-adherent and non-adherent cord blood-derived HSPCs and to exclude that cell cycle and differentiation affect adhesive capacity.

2016-05-16 | GSE78216 | GEO

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