Project description:Peniophora incarnata T-7 is a white-rot fungus with a pronounced capacity for preferential lignin degradation. To investigate the molecular responses of this strain during lignocellulose degradation, P. incarnata T-7 was cultured on different lignocellulosic substrates, including poplar wood, Chinese fir wood, and rice straw, with PDA medium as the control. RNA-seq was performed to characterize substrate-induced gene expression profiles and to identify genes potentially involved in lignin, cellulose, and hemicellulose degradation. This dataset provides transcriptomic resources for understanding the lignocellulose-degrading mechanism and substrate-adaptive responses of P. incarnata T-7.
Project description:Peniophora incarnata T-7 is a white-rot fungus with a strong capability for lignocellulose degradation, particularly lignin-rich substrates. To investigate protein-level responses during lignocellulose degradation, P. incarnata T-7 was cultured on four solid-state conditions, including poplar wood (PW), Chinese fir wood (FW), rice straw (RS), and potato dextrose agar (PDA) as the control. All substrates were cultivated for 30 days under solid-state culture conditions. Label-free quantitative proteomic analysis was performed to characterize substrate-induced protein expression profiles and to identify proteins involved in lignocellulose degradation and metabolic adaptation. Proteins were extracted from fungal mycelia, digested with trypsin, and analyzed using LC-MS/MS mass spectrometry. Raw mass spectrometry data were processed using standard database search workflows for protein identification and label-free quantification.
Project description:Laparoscopic colon surgery has been performed widely. As a minimal invasive approach, single incision (Embryonic NOTES) colon surgery has been attempted.
However, there are not sufficient evidences for single incision colon surgery in colon cancer.
The investigators are researching the efficacy and safety of single incision laparoscopic sigmoidectomy in sigmoid colon cancer.
