West Virginia streams eDNA survey
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB37092 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR3976030_1.fastq.gz | Fastqsanger.gz | |||
| ERR3976030_2.fastq.gz | Fastqsanger.gz | |||
| ERR3976040_1.fastq.gz | Fastqsanger.gz | |||
| ERR3976040_2.fastq.gz | Fastqsanger.gz | |||
| ERR3976050_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 828 |
Similar Datasets
Project description:This SuperSeries is composed of the SubSeries listed below.
2015-12-31 | GSE75658 | GEO
Project description:Biodiversity monitoring of a lake ecosystem through eDNA metabarcoding
| PRJEB13009 | ENA
Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
2015-12-31 | E-GEOD-75658 | biostudies-arrayexpress
Project description: Not available
| S-BSST1405 | biostudies-other
Project description:Environmental monitoring for pharmaceuticals and endocrine disruptors in the aquatic environment traditionally employs a variety of methods including analytical chemistry, as well as a variety of histological and biochemical endpoints that correlate with the fish fitness. It is now clear that analytical chemistry alone is insufficient to identify aquatic environments that are compromised because these measurements do not identify the biologically available dose. The biological endpoints that are measured are important because they relate to known impairments, however, they are not specific to the contaminants and often focus on only a few known endpoints. These studies can be enhanced by looking more broadly at changes in gene expression, especially if the analysis focuses on biochemical pathways. The present study was designed to obtain additional information on well-characterized sites adjacent to sewage treatment plants in MN that are thought to be impacted by endocrine disruptors. Here we examine five sites that have been previously characterized and examine changes in gene expression in fathead minnows (Pimephales promelas) that have been caged for 48 h in each of the aquatic environments. We find that the gene expression changes are characteristic and unique at each of the five sites. Also, fish exposed to two of the sites, 7 and 12, display a more aggressive behavior compared to control fish. Our results show that short-term exposure to sewage treatment plant effluents was able to induce a site-specific gene expression pattern in the fathead minnow gonad and liver. The short-term exposure was also sufficient to affect fish sexual behavior. Our results also show that microarray analysis can be very useful for determining potential exposure to chemicals, and could be used routinely as a tool for environmental monitoring.
2009-06-19 | GSE16645 | GEO
Project description:The relative influence of seasonal patterns in hydrological flow and seasonal differences in biological and geochemical activity on stream chemistry patterns is difficult to discern because they covary; temperate systems are characterized by lower mean flow in the summer (i.e. corresponding to deeper flow paths, elevated temperature, and biological activity), and higher mean flow in the winter (i.e. corresponding to shallower flow paths, depressed temperature, and biological dormancy). Using 2018 data, when seasonal stream flow conditions reversed, and two prior conventional water years, the relationship between monthly acid-relevant analyte concentrations and streamflow were compared within and between winter and summer to provide insight into controls on characteristic seasonal chemistry patterns at two mid-Appalachian sites with distinct geology (weatherable mafic and weather resistant siliciclastic). Acid neutralizing capacity (ANC) increased (1) with lower flow, in both seasons and (2) in summer, for all flow conditions. The compounding impacts resulted in a doubling of concentration from typical winter with high flow to summer with low flow at both sites. Base cation patterns tracked ANC at the mafic site, resulting in an ~ 60% increase of from winter with high flow to summer with low flow; distinctions between summer and winter contributed more to the seasonal pattern (72%) than changes in flow. Sulfate increased at the mafic site (1) with higher flow, in both seasons and (2) in winter, for all flow conditions, resulting in an ~ 50% increase from summer with low flow to winter with high flow; distinctions between winter and summer conditions and flow contributed similarly (40-60%) to the typical seasonal chemical pattern. The biogeochemical mechanism driving differences in stream chemistry between summer and winter for the same flow conditions is likely increased rates of natural acidification from elevated soil respiration in summer, resulting in greater bedrock weathering and sulfate adsorption. Findings highlight the significance and consistency of growing vs dormant season variations in temperature and biological activity in driving intra-annual patterns of stream solutes. This data set informs parameterization of hydro-biogeochemical models of stream chemistry in a changing climate at a biologically relevant, seasonal, timescale.Supplementary informationThe online version contains supplementary material available at 10.1007/s10533-024-01163-x.
| S-EPMC11489209 | biostudies-literature
Project description:Development of an Environmental Metagenetic Approach for Monitoring Aquatic Biodiversity
| PRJNA317862 | ENA
Project description:Coal is a major energy source that generates diverse environmental impacts through its production, primarily by the release of coal dust particles. An aqueous coal dust extract was obtained from a mineral sample taken from one of the largest coal mines in Colombia (La Loma, Cesar), trace elements by ICP/MS were measured, and its toxicity evaluated using the zebrafish (Danio rerio) vertebrate model. In this study, zebrafish embryos were exposed to different concentrations of aqueous coal extract (0, 0.1, 1, 10, 100 and 1000 parts per million (ppm; μg/mL) to establish acute toxicity, as well as morphological and transcriptome alterations. Trace elements within the coal extract yielding the highest concentrations included Sr, Zn, Ba, As, Cu, Se, Li, Ni, Sb, Rb, Co, and Cr. In addition, Cd and Pb were found in lower concentrations. No significant difference in mortality was observed with survival near 90% in all treatments. A significant decrease in rate of hatching was observed in the 0.1 and 1000 ppm treatment groups at 72 hpf. Furthermore, no significant differences in total body length, head length, or head diameter was observed in any of the treatment groups. Transcriptomic results of zebrafish larvae revealed alterations in 77, 61, and 1,376 genes in the 1, 10, and 100 ppm treatments, respectively. Gene ontology analysis revealed gene alterations associated with hematological system development and function, tissue morphology and development, connective tissue development and function, and embryonic development. Overall, these findings are the first to identify gene expression alterations in response to a developmental aqueous coal dust residue from coal mining.
2017-11-30 | GSE94997 | GEO
Project description:Coal mining carries inherent risks of catastrophic gas explosions capable of inflicting severe lung injury. Using a rat model, we explored mechanisms underlying alveolar epithelial damage and repair following a gas explosion. By performing scRNA-sequencing, we revealed that alveolar epithelial cells exhibited the most profound transcriptomic changes after gas explosion compared to other pulmonary cell types.
2024-01-18 | GSE252844 | GEO