Similar Datasets
Project description:To support our molecular hypothesis of the effect of rpoS and inrR genes over the activation of the ICEclc genome in Pseudomonas knackmussii B13, we have employed Agilent DNA custom microarray in the 8X15K format.
2012-09-01 | GSE35501 | GEO
Project description:In this research a high-throughput RNA sequencing based transcriptome analysis technique (RNA-Seq) was used to evaluate differentially expressed genes (DEGs) in the wild type Arabidopsis seedling in response to flg22, a well-known microbe-associated molecular patterns (MAMP), and AtPep1, a well-known peptide representing an endogenous damage-associated molecular patterns (DAMP). The results of our study revealed that 1895 (1634 up-regulated and 261 down-regulated) and 2271 (1706 up-regulated and 565 down-regulated) significant differentially expressed genes in response to flg22 and AtPep1 treatment, respectively. Among significant DEGs, we observed that a number of hitherto overlooked genes have been found to be induced upon treatment with either flg22 or with AtPep1, indicating their possible involvement in innate immunity. Here, we characterized two of them, namely PP2-B13 and ACLP1. PP2-B13 contains an F-box domain and shows similarity to carbohydrate binding proteins. ACLP1 is a protein of unknown function with highest similarity to actin cross linking proteins and includes a fascin domain. Using qPCR, we verified that the genes encoding PP2-B13, and ACLP1 were highly induced upon treatment of leaf disks with flg22. We obtained T-DNA insertion mutants and generated homozygous mutant lines. None of the mutants showed a phenotype in the absence of infection. pp2-b13 and aclp1 mutants showed an increased susceptibility to infection by the virulent pathogen Pseudomomas syringae pv tomato mutant hrcC-, as evidenced by an increased growth of the pathogen in planta. Further we present evidence that aclp1 was deficient in ethylene production upon flg22 treatment, while pp2-b13, was deficient in ROS production. In conclusion, the products of these genes contribute to plant immunity against bacterial pathogens, although there is currently no clue for their mechanism of action. The results from this research provide new information to a better understanding of the immune system in Arabidopsis.
2020-12-11 | E-MTAB-9838 | biostudies-arrayexpress
Project description:bacterium B13(2017) isolate:B13 Genome sequencing and assembly
| PRJNA360183 | ENA
Project description:To support our molecular hypothesis of the effect of rpoS and inrR genes over the activation of the ICEclc genome in Pseudomonas knackmussii B13, we have employed Agilent DNA custom microarray in the 8X15K format. ICEclc-specific gene expression in Pseudomonas knackmussii strain B13 was measured after 48 hours of stationary phase following a growth on 10mM 3-chlorobenzoate (3CBA) as sole carbon and energy source. Various genotypes were tested: wild type (strain nM-BM-078, in 5 replicates), inrR-/- (strain 2201, in triplicates), delta::rpoS (strain 2671, in triplicates).
2012-09-01 | E-GEOD-35501 | biostudies-arrayexpress
Project description:Mouse 129-B13 ESCs were genetically modified using CRISPR-Cas9 with two guides to remove exon 5 of Phf14, single-cell sorted and expanded to establish modified cell lines (2). Samples were differentiated from mouse ESCs (Day 0) to embryoid bodies (Day 4), neural progenitor cells (Day 8) and glutamatergic neurons (Day 12). Additionally, the parental 129-B13 ESC wild-type cells were split into two and differentiated separately as controls. NEBNext Poly(A) mRNA Magnetic Isolation Module was used to enrich mRNA. Nonsense-mediated decay was observed in Phf14 ex 5 KOs and no Phf14 protein expression was detected by western blot in these cells.
2023-02-01 | E-MTAB-10491 | biostudies-arrayexpress
Project description:By using oligonucleotide microarray-based technology as a tool for rapid mapping of unique differences in gene expression, we found that inhibiting serpin B13 in BALB/c mice treated with a mAb according to our protocol resulted in the upregulation of relatively few genes, including several Reg genes, which have been implicated in β-cell proliferation.
2019-03-07 | GSE125151 | GEO
Project description:WGS of Enterococcus faecium B13
| PRJNA1029823 | ENA