Project description:Investigation of whole genome gene expression level changes in Candida glabrata CBS138 delta-vph2 mutant, compared to the wild-type strain in SC broth (pH5.0 and pH7.4). VPH2 gene encodes a protein that is the assembly factor of a functional V-ATPase. Loss of Vph2p leads to loss of a functional V-ATPase enzyme complex.
Project description:To estiamte the role lipid flippase subunit Cdc50 plays in Candida glabrata, a Δcdc50 null mutant was constructed based on standard strain ATCC2001 (wild type, WT) through homologous recombination. Then, gene expression profiling analysis was performed using data obtained from RNA-seq of WT and Δcdc50 mutant.
Project description:To estiamte the role lipid flippase gene Drs2 plays in Candida glabrata, a ΔDrs2 null mutant was constructed based on standard strain ATCC2001 (wild type, WT) through homologous recombination. Then, gene expression profiling analysis was performed using data obtained from RNA-seq of WT and ΔDrs2 mutant.
Project description:Homo sapiens fresh whole blood was infected with Candida glabrata. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Measurement of Candida glabrata gene expression.
Project description:Comparison of young and old Candida glabrata cells reveals regulation of genes involved in cell wall remodeling, sterol biogenesis, stress pathways
Project description:Several aneuploid Candida glabrata strains, each bearing a unique karyotype, were spread on YPD plates. They all exhibited colony size vairations. Randmly 10 large colonies from each strain were sequenced.
