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RNA-Seq of human AML cell line, HL-60 and its mitoxantrone resistant subline HL60/MX2

ABSTRACT: RNA-Seq of human AML cell line, HL-60 and its mitoxantrone resistant subline HL60/MX2

PROVIDER: PRJEB37958 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4056990_1.fastq.gz	Fastqsanger.gz
	ERR4056990_2.fastq.gz	Fastqsanger.gz
	ERR4056987_1.fastq.gz	Fastqsanger.gz
	ERR4056987_2.fastq.gz	Fastqsanger.gz
	ERR4056988_1.fastq.gz	Fastqsanger.gz

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RNA-Seq of human AML cell line, HL-60 and its mitoxantrone resistant subline HL60/MX2

Project description:Alteration in metabolic repertoire is commonly associated with resistance phenotype. Although it’s a common phenotype, not much efforts have been undertaken to design effective strategies to target the metabolic drift in such cancerous cells and especially with drug resistant properties. In our study, we identified that drug resistant AML cell line HL-60/MX2 do not follow classical Warburg effect, instead these cells exhibit drastically low levels of aerobic glycolysis. Biochemical analysis confirms reduced glucose consumption and lactic acid production by resistant population with no differences in glutamine consumption. Raman spectroscopy revealed increased lipid and cytochrome content in resistant cells which were also visualized in the form of lipid droplets by Raman mapping, electron microscopy and lipid specific staining. Gene set enrichment analysis data from both the cell lines revealed significant enrichment of lipid metabolic pathways in HL-60/MX2 cells. Further drug resistant cells possess higher mitochondrial activity and increased OXPHOS suggested the role of fatty acid metabolism as energy source which was confirmed by increased rate of fatty acid oxidation. Pharmacological inhibition of fatty acid oxidation using Etomoxir affected the colony formation ability of resistant cells and inhibition of OXPHOS using Antimycin-A increased the sensitivity of resistant cells to chemotherapeutic drug, demonstrating requirement of fatty acid metabolism and increased dependency on OXPHOS by resistant leukemic cells for tumorigenicity.

2021-01-01 | E-MTAB-8981 | biostudies-arrayexpress

mRNA-sequencing in drug-resistant acute myeloid leukemia

Project description:mRNA sequencing for AML cells(HL-60) and drug-resistant AML Cells(HL60/MX2).

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Project description:m7G-MeRIP-sequencing for AML cells(HL-60) and drug-resistant AML Cells(HL60/MX2).

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Gene expression profiles during the process of differentiation of HL-60 cells into neutrophils [HL60]

Project description:Gene expression profiles during the process of differentiation of HL-60 cells into neutrophils [HL60]

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Hi-C detects novel rearrangements in HL60 and HL60/S4 cell lines

Project description:Hi-C detects novel rearrangements in HL60 and HL60/S4 cell lines

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RNA-seq analysis of neutrophils from CD40L-deficient patients and healthy controls, as well as HL-60 promielocytic cell line

Project description:We aim to characterize to effects of the absence of CD40L on neutrophil transcriptome and the effect of soluble CD40L on HL60 cells. For this purpose Total RNA of isolated neutrophils from three CD40L-deficient patients and three healthy controls as well as HL-60 cells from ATCC (HL-60 (ATCC CCL-240) were analyzed by RNAseq. Before RNA obtention, neutrophils were incubated for 2 hours in the presence or absence of 100 U/ml rhIFN- (Immukine, Boehringer Ingelheim), and HL-60 cells cultured for 6 days in the presence or absence of 500 ng/mL sCD40L and/or 1,0 % dimethyl sulfoxide (DMSO).

2017-03-14 | E-MTAB-5316 | biostudies-arrayexpress

Dietzia maris strain:MX2

Project description:Dietzia maris strain:MX2 Genome sequencing and assembly

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Effects of supernatants of USP2 knockdown HL-60 cells and mock transfected HL60 cells on differentiated 3T3-L1 cells

Project description:Ubiquitin specifc protease (USP) 2 modulates various cellular responces including carcinogenesis, spermatogenesis, and glucose metabolism. So far, we established USP2 knockdown HL-60 cells to explore roles of USP2 in macrophages. In this experiment, we investigated whether USP2 knockdown HL-60 cells more prominently modulate function of adipocytes compared with control cells.

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Homo sapiens

Project description:RNA-seq for siPRKDC and siControl in human HL-60/MX2 mitoxantrone resistant derivative of the HL-60 cell line.

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comparison of HL60-NG cell and HL60-RG cell

Project description:Human Promyelocytic Leukemia Cell Line HL60 has been used extensively as a model for myeloid differentiation and leukemia. HL60-RG cell is its sub-line and shows a higher growth rate. In this study, we carried out a genome scan using SNP 10k mapping array on both cells. Comparative study on chromosomal changes of these cells will give us information on mechanism of tumor progression. Keywords: cell type comparison

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