Project description:Comparison of gene expression profiles from Danio rerio brain of wiltype (AB) and homozygote/heterozygote tert mutants (AB tert(-/-), AB tert(+/-)). The RNA-seq data comprise 3 groups. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:Comparison of gene expression profiles from Danio rerio muscle of wiltype (AB) and homozygote/heterozygote tert mutants (AB tert(-/-), AB tert(+/)). The RNA-seq data comprise 3 groups. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:Comparison of gene expression profiles from Danio rerio liver of wiltype (AB) and homozygote/heterozygote tert mutants (AB tert(-/-), AB tert(+/)). The RNA-seq data comprise 3 groups. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:Comparison of gene expression profiles from Danio rerio skin of wiltype (AB) and homozygote/heterozygote tert mutant phenotypes (AB tert(-/-), AB tert(+/)). The RNA-seq data comprises 3 groups. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:Strains of commonly used wildtype zebrafish (Danio rerio) have both shared and unique genomic features such as SNPs and copy number variants. Unfortunately, exact lab strains are often unreported in the literature and the replication of studies in zebrafish may be negatively impacted by this fact. These data were collected to assess hepatic mRNA expression patterns between three common zebrafish strains (AB, Tuebingen, and WIK) to establish baseline differences across strains and between sexes.Baseline mRNA expression was analyzed in six individuals pers strain (three males and three females) using Agilent 4x44K gene expression microarrays . These data highlight the complexity of variation that exists within zebrafish and underscore the value of this model system as a valid representation of normal variation present in other species, including humans.
Project description:Proteins expressed and exported out of the mycobacterial biofilm cells into granulomatous environment during infection were identified by LC-MS/MS. To this end, adult 5- to 10-month-old female AB wild-type zebrafish (Danio rerio) were infected with 4-day old M. marinum ATCC927 (carrying the pTEC27 plasmid expressing the tdTomato fluorescent protein) cells. After 8 weeks post infection, ten zebrafish were euthanized, and red fluorescent mycobacterial granulomas were carefully dissected from the zebrafish ovaries. Ten granulomas were collected per tube, frozen on dry ice and stored at -80 °C until preparation for proteomics. Altogether, ten replicate samples, each with ten mycobacterial granulomas, were subjected to protein extraction and LC-MS/MS identification.
