Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

STEC new stx subtype

ABSTRACT: STEC new stx subtype

PROVIDER: PRJEB39088 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	ERR4297034.fastq.gz	Fastqsanger.gz
	ERR4297035.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

New Stx subtypes

Project description:New Stx subtypes

| PRJEB38687 | ENA

The probiotic Escherichia coli strain Nissle 1917 combats the lambdoid bacteriophages stx and λ

Project description:The Escherichia coli strain Nissle 1917 (EcN) is used as a probiotic for the treatment of certain gastrointestinal diseases in several European and non-European countries. In vitro studies showed EcN to efficiently inhibit the production of Shiga toxin (Stx) by Stx producing E. coli (STEC) such as Enterohemorrhagic E. coli (EHEC). The occurrence of the latest EHEC serotype (O104:H4) responsible for the great outbreak in 2011 in Germany was due to the infection of an enteroaggregative E. coli by a Stx 2-encoding lambdoid phage turning this E. coli into a lysogenic and subsequently into a Stx producing strain. Since EHEC infected persons are not recommended to be treated with antibiotics, EcN might be an alternative medication. However, because a harmless E. coli strain might be converted into a Stx-producer after becoming host to a stx encoding prophage, we tested EcN for stx-phage genome integration. Our experiments revealed the resistance of EcN towards not only stx-phages but also against the lambda phage. This resistance was not based on the lack of or by mutated phage receptors. Rather the expression of certain genes (superinfection exclusion B (sieB) and a phage repressor (pr) gene) of a defective prophage of EcN was involved in the complete resistance of EcN to infection by the stx- and lambda phage. Obviously, EcN cannot be turned into a Stx producer. Furthermore, we observed EcN to inactivate phages and thereby to protect E. coli K-12 strains against infection by stx- as well as lambda-phages. Inactivation of lambda-phages was due to binding of lambda-phages to LamB of EcN whereas inactivation of stx-phages was caused by a thermostable protein of EcN. These properties together with its ability to inhibit Stx production make EcN a good candidate for the prevention of illness caused by EHEC and probably for the treatment of already infected people.

2018-06-14 | GSE109932 | GEO

RNA SEQ STEC FHI96 Type III mod

Project description:Comparison of RNA expression profiles from STEC strain FHI96 expressing methyltransferase mod (ON; 52reps) or not (OFF; 51reps).

2024-06-30 | GSE129787 | GEO

Expression data from mouse kidney intraperitoneally administered with LPS and Stx

Project description:Mice intraperitoneally administered with LPS and Stx exhibit HUS-like pathology. While mouse and human Gb3 localization is different, LPS and Stx induced kidney injury models in mice have been used to confirm responsiveness to various stx-related inflammatory pathways and treatments. In order for this mouse model to apply tHUS in humans, more detailed and exhaustive comprehension of this animal model is needed. Although molecular studies have been conducted on this mouse model before, we consider that there is still scope for further investigation of molecular pathways and studies on kidney damage segments. Overall, Biological pathways, upstream regulators, and downstream biological activities occurring in the kidney after LPS/Stx administration were identified through Ingenuity Pathway Analysis ™ using the result of microarray. In addition, we identified the detailed damaged site in the renal tubule from the down-regulation gene revealed by microarray.

2021-09-01 | GSE180476 | GEO

STX-LST

Project description:Loss of stx from Escherichia coli and Escherichia albertii

| PRJEB27634 | ENA

4D-FastDIA quantitative phosphorylation modification omics analysis before and after expression of Salmonella effector SteC in RAW264.7 cell

Project description:The Salmonella effector SteC is the only protein kinase encoded by Salmonella pathogenicity island 2 that is secreted through the type III secretion system. SteC is known to trigger actin rearrangement via the phosphorylated MEK pathway, and our previous experiments demonstrated that the migration process of macrophages found during Salmonella infection is dependent on the rearrangement of the host cell actin backbone and the action of SteC.To further investigate the target of SteC in the host, we constructed a SteC-RAW264.7 cell line and performed phosphomics analysis using 4D-FastDIA to identify the direct substrates of SteC that trigger macrophage migration and lead to cytoskeletal rearrangement.

2024-02-22 | PXD042038 | Pride

Escherichia coli strain:STEC | isolate:STEC

Project description:Escherichia coli strain:STEC | isolate:STEC Genome sequencing

| PRJNA1435770 | ENA

Prevalence and distribution of the stx, stx genes in Shiga toxin producing E. coli (STEC) isolates from cattle.

Project description: Not available

| S-EPMC3279763 | biostudies-literature

Effect of Shiga toxin (Stx) and glucosylceramide synthase inhibitor (Gb3i) treatments on SV40 transformed human kidney endothelial cells

Project description:To investigate the transcriptional changes caused by Stx1 and Stx2 in human kidney microvascular cells (HKMECs), and the effects of GCSi pre-treatment on Stx-mediated effects

2024-01-24 | GSE205723 | GEO

Molecular surveillance of STEC

Project description:Routine surveillance of STEC at the Belgian NRC STEC

| PRJEB54005 | ENA