Ontology highlight
ABSTRACT:
PROVIDER: PRJEB39811 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:To elucidate the target genes of ArgR in Aeromonas veronii, we engineered an Aeromonas veronii strain that expresses the ArgR protein fused to a 3× FLAG tag, and FLAG antibodies were employed for the immunoprecipitation of DNA-protein complexes.
2024-09-15 | GSE277129 | GEO
Project description:The intestinal epithelial gene responses to Aeromonas veronii infection and the pathogenic mechanisms were investigated by comparative differential expression analysis
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Project description:The macrophage cell (THP-1 derived macrophages) gene responses to Aeromonas veronii and Escherichia coli were investigated by comparative differential expression analysis
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Project description:The bacterium Aeromonas veronii is a co-pathogenic species that can negatively impact the health of both humans and aquatic animals. In this study, we used single-cell transcriptome analysis (scRNA-seq) to investigate the effects of infection with A. veronii on head kidney cells and the regulation of gene expression in the dark sleeper (Odontobutis potamophila). scRNA-seq was used to assess the effects of infection with A. veronii in O. potamophila B cells, endothelial cells, macrophages, and granulocytes, and differential enrichment analysis of gene expression in B cells and granulocytes was performed. The analyses revealed a significant increase in neutrophils and decrease in eosinophils in granulocytes infected with A. veronii. Activation of neutrophils enhanced ribosome biogenesis by up-regulating the expression of rps12 and rpl12 to fight against invading pathogens. Crucial pro-inflammatory mediators il1b, ighv1-4, and the major histocompatibility class II genes mhc2a and mhc2dab, which are involved in virulence processes, were up-regulated, suggesting that A. veronii activates an immune response that presents antigens and activates immunoglobulin receptors in B cells. These cellular immune responses triggered by infection with A. veronii enriched the available scRNA-seq data for teleosts, and these results are important for understanding the evolution of cellular immune defense and functional differentiation of head kidney cells.
2023-05-16 | GSE229275 | GEO
Project description:Largemouth bass (Micropterus salmoides) were challenged with Aeromonas veronii (Av), Aeromonas hydrophila (Ah), Flavobacterium columnare (Fc), and coinfections (AvFc, AhFc), alongside mock-challenged and pre-challenge controls. Gill and spleen tissues were sampled at 0h (pre-challenge baseline), 12h, and 24h post-challenge. RNA-Seq was performed on 161 samples (79 gill, 82 spleen; 6 biological replicates per treatment/timepoint group, 10 for pre-challenge). Three gill samples were excluded due to low mapping rates consistent with gDNA contamination (11B3G, 15J2G, 29R3G). Differential expression analysis was performed using DESeq2 across 72 pairwise contrasts per tissue.
2026-07-31 | GSE333434 | GEO
Project description:Aeromonas caviae has been associated with human gastrointestinal disease. Strains of this species typically lack virulence factors (VFs) such as enterotoxins and hemolysins that are produced by other human pathogens of the Aeromonas genus. Microarray profiling of murine small intestinal extracts, 24 hours after oral infection with an A. caviae strain, provides evidence of a Th1 type immune response. A large number of gamma-interferon (γ-IFN) induced genes are up-regulated as well as several tumor necrosis factor-alpha (TNF-α) transcripts. A. caviae has always been considered an opportunistic pathogen because it lacks obvious virulence factors. This current effort suggests A. caviae colonizes murine intestinal tract and causes what has been described by others as a dysregulatory cytokine response leading to an irritable bowel-like syndrome. This response would explain why a number of diarrheal waterborne outbreaks have been attributed to A. caviae even though it lacks obvious enteropathogenic properties. Keywords: Aeromonas caviae, infection, disease mechanism, TH1 resposne
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