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Transcriptome of holcus lanatus

ABSTRACT: transcriptome of holcus lanatus

PROVIDER: PRJEB3994 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR294007_1.fastq.gz	Fastqsanger.gz
	ERR294007_2.fastq.gz	Fastqsanger.gz
	ERR294008_1.fastq.gz	Fastqsanger.gz
	ERR294008_2.fastq.gz	Fastqsanger.gz
	ERR294009_1.fastq.gz	Fastqsanger.gz

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RNAseq study (root and shoot) of Holcus lanatus ecotypes selected from two widely contrasting habitats, acid bog (pH 3.5) or limestone quarry spoil (pH 7.5)

Project description:The aim of this study was to investigate ecotypic adaptation in Holcus lanatus in plants selected from two widely contrasting habitats, acid bog (pH 3.5) or limestone quarry spoil (pH 7.5), using a transcriptome based analysis approach including sequence analysis of root associated Glomeromycota. Differential gene expression in root and shoot of naturally occurring H. lanatus ecotypes, selected from either habitat and grown in a full factorial reciprocal soil transplant experiment were investigated and ecotype specific SNPs identified.

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Project description:The wild grass Holcus lanatus L., an outcrossing diploid (2n=14) and closely related to B. distachyon (Aliscioni et al., 2012), has a remarkable balanced polymorphism in arsenate tolerance, screened from a semi-natural, non-arsenic contaminated populations (Meharg et al., 1993), coded by a single gene (Macnair et al., 1992). As arsenate is a phosphate analogue it has been postulated that this polymorphism is maintained due to phosphorus nutrition, not arsenate tolerance per se, particularly as the tolerance gene co-segregates with suppression of High affinity Phosphate Transport (HAPT) (Meharg et al., 1992a; Meharg & Macnair, 1992b), though an explicit ecological link to phosphorus status of soils has yet to be proven (Naylor et al., 1996). The aim of this study is to address soil phosphate responsiveness (+/-) along with the transcriptomic consequences of being of arsenic tolerant (T) or non-tolerant (N) phenotype to ascertain why and how this polymorphism is maintained.

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