LH244 Bayer
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB40019 | ENA |
REPOSITORIES: ENA
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Project description:Development of crop varieties with high nitrogen use efficiency (NUE) is crucial for minimizing N loss, reducing environmental pollution and decreasing input cost. Maize is one of the most important crops cultivated worldwide and its productivity is closely linked to the amount of fertilizer used. A survey of the transcriptomes of shoot and root tissues of a maize hybrid line and its two parental inbred lines grown under sufficient and limiting N conditions by mRNA-Seq has been conducted to have a better understanding of how different maize genotypes respond to N limitation.
2014-01-09 | E-MTAB-4410 | biostudies-arrayexpress
Project description:In maize crops Southern Leaf Blight (SLB) is a severe disease caused by the fungus Bipolaris maydis (Y. Nisik & C. Miyake) leading to several losses in crop production. Unlike in maize, popcorn resistant varieties are not yet commercially available and this trait have been the subject of many popcorn breeding programs. The present study aimed to identify differentially accumulated proteins (DAPs) associated with resistance to B. maydis in two contrasting popcorn inbred lines using the comparative proteomic analysis. Forty-day old popcorn plants from resistant and susceptible inbred lines at the V4 growth stage were inoculated with B. maydis by spraying the conidium suspension. At four and ten days after inoculation, the morphological aspects of lesions in leaves were checked and the proteomic analysis was performed. Our results showed that the resistant inbred line exhibited minor foliar lesions in comparison with the SLB-susceptible genotype. In the genotype’s comparison, 644 DAPs were identified at 4 days and 613 at 10 days after B. maydis inoculation. Besides that, in resistant plants were identified DAPs upaccumulated related to response to stress, response to stimulus, photosynthesis, cellular growth, maintenance and detoxification process, which might be involved in plant response against the pathogen. It was identified proteins as salicylic acid-related and nucleotide-binding leucine-rich repeat that may be responsible to initiate a response to fungus in SBL-resistant inbred line. A differential response was identified in chloroplast proteins that is the main organelle involved in perception of the fungus to initiate a signalizing response. The changes in the proteomic profile in resistant inbred line may be effective in the response under B. maydis infection. This is the first work presenting the proteomic profile alteration under B. maydis inoculation and these findings may be useful for identifying candidate biomarkers in popcorn resistance to B. maydis being relevant for further researches on genetic breeding to develop resistant genotypes.
2025-02-03 | PXD016768 | Pride
Project description:Aluminum toxicity is one of the most important abiotic stresses that affects crop production worldwide. The soluble form (Al3+) inhibits root growth, altering water and nutrients uptake reducing the plant growth and development. Under a long term of Al3+ exposure, plants can activate several tolerance mechanisms and, to date, there are no reports of large-scale proteomic data of maize in response to this ion. To investigate the post-transcriptional regulation in response to Al toxicity, we performed a label-free quantitative proteomics for comparative analysis of two Al-contrasting popcorn inbred lines and an Al-tolerant commercial hybrid during 72 h under Al-stress. A total of 489 differentially accumulated proteins (DAPs) were identified in the Al-sensitive inbred line, 491 in the Al-tolerant inbred line, and 277 in the commercial hybrid. Among then, 120 DAPs were co-expressed in both Al tolerant genotypes. Bioinformatics analysis indicated that starch and sucrose metabolism, glycolysis/gluconeogenesis, and carbohydrate metabolism were significant biochemical process regulated in response to Al toxicity. The up-accumulation of sucrose synthase and the increased of sucrose content and starch degradation suggest that these components may be enhance popcorn tolerance to Al stress. The up-accumulation of citrate synthase suggests a key role of this enzyme in the detoxification process in the Al-tolerant inbred line. The integration of transcriptomic and proteomic data indicated that Al tolerance response presents a complex regulatory network into the transcription and translation dynamics of popcorn roots development.
2023-05-22 | PXD027588 | Pride
Project description:Head smut of maize, which is caused by the Sporisorium reilianum f. sp. Zeae (Kühn), has been a serious disease in maize. In order to find head smut resistant candidate genes, microarrays were used to monitor the gene expression profiles between disease resistant near isogenic lines (NIL) L282 and L43, highly resistant inbred line Q319 and highly susceptible inbred line Huangzao4 after 0 to7 days post inoculation of S.reiliana by artificial inoculation method.
2012-08-16 | GSE40052 | GEO
Project description:RNA-seq from whole V3 maize seedlings (roots and shoots) for the B73 inbred line and A188 x B73 hybrid.
2022-01-27 | GSE175914 | GEO
Project description:We isolated fixed maize anther cells for single-cell RNA-seq. All anthers were 2.0 mm from the W23 inbred line.
2021-09-07 | GSE183422 | GEO
Project description:We demonstrated the manifestation of heterosis in hybrid maize embryo and endosperm tissue six days after fertilization in crosses of several inbred lines. Here we analyzed heterosis-associated gene expression pattern in these tissues of reciprocal crosses of two european maize inbred line combinations. Differences in gene expression were analyzed with custom microarrays by a combined approach of suppression subtractive hybridization and microarray hybridizations
2009-08-28 | GSE17821 | GEO
Project description:We sequenced and analyzed the genome of a highly inbred miniature Chinese pig strain, the Banna Minipig Inbred Line (BMI). we conducted whole genome screening using next generation sequencing (NGS) technology and performed SNP calling using Sus Scrofa genome assembly Sscrofa11.1.
2020-12-31 | GSE157688 | GEO