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Principle experiment

ABSTRACT: Linkages between decomposition of terrigenous dissolved organic matter (DOM) and microbial community structure: influences of chemical DOM characteristics.

PROVIDER: PRJEB4004 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR295073.fastq.gz	Fastqsanger.gz
	ERR295074.fastq.gz	Fastqsanger.gz
	ERR295075.fastq.gz	Fastqsanger.gz
	ERR295076.fastq.gz	Fastqsanger.gz
	ERR295077.fastq.gz	Fastqsanger.gz

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DOM composition changes in a warming peatland

Project description:Measure changes in dissolved organic matter composition and resulting microbial decomposition rates in an experimentally warmed peatland.

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Microbial community functioning during plant litter decomposition

Project description:Microbial life in soil is fueled by dissolved organic matter (DOM) that leaches from the litter layer. It is well known that decomposer communities adapt to the available litter source, but it remains unclear if they functionally compete or synergistically address different litter types. Therefore, we decomposed beech, oak, pine and grass litter from two geologically distinct sites in a lab-scale decomposition experiment. We performed a correlative network analysis on the results of direct infusion HR-MS DOM analysis and cross-validated functional predictions from 16S rRNA gene amplicon sequencing and with DOM and metaproteomic analyses. Here we show that many functions are redundantly distributed within decomposer communities and that their relative expression is rapidly optimized to address litter-specific properties. However, community changes are likely forced by antagonistic mechanisms as we identified several natural antibiotics in DOM. As a consequence, the decomposer community is specializing towards the litter source and the state of decomposition (community divergence) but showing similar litter metabolomes (metabolome convergence). Our multi-omics-based results highlight that DOM not only fuels microbial life, but it additionally holds meta-metabolomic information on the functioning of ecosystems.

2024-02-14 | MTBLS1885 | MetaboLights

Project description:Effects of terrigenous dissolved organic matter (tDOM) input in coastal waters.

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Project description:Non-targeted Metabolomics from CCE2024 Dissolved Organic Matter (DOM) samples analyzed in positive mode

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Project description:Transformation of terrigenous dissolved organic matter along a salinity gradient predator free mesocosms

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Project description:Effects of terrigenous dissolved organic matter (tDOM) and temperature increase in coastal waters.

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Using network analysis to discern compositional patterns in ultrahigh resolution mass spectrometry data of dissolved organic matter

Project description:RATIONALE: Marine dissolved organic matter (DOM) has long been recognized as a large and dynamic component of the global carbon cycle. Yet, DOM is chemically varied and complex and these attributes present challenges to researchers interested in addressing questions about the role of DOM in global biogeochemical cycles.METHODS: Organic matter extracts from seawater were analyzed by direct infusion with electrospray ionization into a Fourier transform ion cyclotron resonance mass spectrometer. Network analysis was used to quantify the number of chemical transformations between mass-to-charge values in each sample. The network of chemical transformations was calculated using the MetaNetter plug-in within Cytoscape. The chemical transformations serve as markers for the shared structural characteristics of compounds within complex DOM.RESULTS: Network analysis revealed that transformations involving selected sulfur-containing moieties and isomers of amino acids were more prevalent in the deep sea than in the surface ocean. Common chemical transformations were not significantly different between the deep sea and surface ocean. Network analysis complements existing computational tools used to analyze ultrahigh-resolution mass spectrometry data.CONCLUSIONS: This combination of ultrahigh-resolution mass spectrometry with novel computational tools has identified new potential building blocks of organic compounds in the deep sea, including the unexpected importance of dissolved organic sulfur components. The method described here can be readily applied by researchers to analyze heterogeneous and complex DOM.

2016-10-31 | MTBLS366 | MetaboLights

SynWH7803_DOM

Project description:Dissolved organic matter prepared via PPL SPE from cultures of Synechococcus WH7803. Biological triplicate samples were prepared of DOM released via exudation, mechanical lysis, and viral lysis by phage S-SM1.

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GNPS Environmental MEtabolomics from SEASCAPE Ocean Seaspray Mesocosm Experiment 2019

Project description:Non-targeted LC-MS/MS from SPE (PPL) enriched samples from dissolved organic matter, total organic matter, sea surface microlayer and seaspray aerosols (DOM, TOM, SSML, SSA) from SeaScape 2019.

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Non-targeted Metabolomics of Coastal Dissolved Organic Matter (DOM) from TARA/TREC 2023 Leg 1

Project description:Non-targeted LC-MS/MS analysis of PPL solid phase extracted dissolved organic matter (DOM) from TARA/TREC Expedition Leg 1, collected in the coastal Atlantic between France to Netherlands in Spring 2023.

2023-08-06 | MSV000092604 | MassIVE

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