Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Antibiotic resistance genes and aquaculture environment

ABSTRACT: antibiotic resistance genes and aquaculture environment

PROVIDER: PRJEB40530 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR4627010.fastq.gz	Fastqsanger.gz
	ERR4627020.fastq.gz	Fastqsanger.gz
	ERR4627030.fastq.gz	Fastqsanger.gz
	ERR4627040.fastq.gz	Fastqsanger.gz
	ERR4627050.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 56

Similar Datasets

Dissemination of antibiotic resistance genes

Project description:Dissemination of antibiotic resistance genes

| PRJEB44153 | ENA

Detection of antibiotic resistance genes in human gut microbiota

Project description:We used a DNA microarray chip covering 369 resistance types to investigate the relation of antibiotic resistance gene diversity with humansM-bM-^@M-^Y age. Metagenomic DNA from fecal samples of 123 healthy volunteers of four different age groups, i.e. pre-school Children (CH), School Children (SC), High School Students (HSS) and Adults (AD) were used for hybridization. The results showed that 80 different gene types were recovered from the 123 individuals gut microbiota, among which 25 were present in CH, 37 in SC, 58 in HSS and 72 in AD. Further analysis indicated that antibiotic resistance genes in groups of CH, SC and AD can be independently clustered, and those ones in group HSS are more divergent. The detailed analysis of antibiotic resistance genes in human gut is further described in the paper DNA microarray analysis reveals the antibiotic resistance gene diversity in human gut microbiota is age-related submitted to Sentific Reports The antibiotic resistance gene microarray is custom-designed (Roche NimbleGen), based on a single chip containing 3 internal replicated probe sets of 12 probes per resistance gene, covering the whole 315K 12-plex platform spots.

2014-01-16 | E-GEOD-54070 | biostudies-arrayexpress

Detection of antibiotic resistance genes in human gut microbiota

Project description:We used a DNA microarray chip covering 369 resistance types to investigate the relation of antibiotic resistance gene diversity with humans’ age. Metagenomic DNA from fecal samples of 123 healthy volunteers of four different age groups, i.e. pre-school Children (CH), School Children (SC), High School Students (HSS) and Adults (AD) were used for hybridization. The results showed that 80 different gene types were recovered from the 123 individuals gut microbiota, among which 25 were present in CH, 37 in SC, 58 in HSS and 72 in AD. Further analysis indicated that antibiotic resistance genes in groups of CH, SC and AD can be independently clustered, and those ones in group HSS are more divergent. The detailed analysis of antibiotic resistance genes in human gut is further described in the paper DNA microarray analysis reveals the antibiotic resistance gene diversity in human gut microbiota is age-related submitted to Sentific Reports

2014-01-16 | GSE54070 | GEO

Dissemination of size-fractionated antibiotic resistance genes

Project description:Dissemination of size-fractionated antibiotic resistance genes

| PRJEB46883 | ENA

soil antibiotic resistance genes and carcass decomposition

Project description:soil antibiotic resistance genes and carcass decomposition

| PRJEB41464 | ENA

Antibiotic resistance in Aeromonas hydrophila associated with exposure to low levels of oxytetracycline

Project description:Antibiotic resistance (AMR) in aquatic bacteria affecting aquaculture has been a growing concern given the potential for mixing of bacterial populations in the aquatic environment and exposure to different pharmaceuticals from drugs used in aquaculture, as well as wastewater effluent and agricultural run-off. To better understand the mechanism for AMR in a common aquatic fish pathogen exposed to low dose antibiotics we monitored the genetic changes, as well as gene expression, in Aeromonas hydrophila as the bacteria was exposed to incremental doses of oxytetracycline (OTC), a commonly used drug in aquaculture. We were able to render all three isolates of our original A. hydrophila resistant to therapeutic levels of OTC (i.e. ≥100ppm). The relatively quick phenotypic adaptation (often less than 3 days) to different OTC concentrations was very similar across our replicates. Our whole genome sequencing data and transcriptome results suggested several genes underwent point mutations across all replicates. Further differential gene expression was observed and likely impacted several pathways which may explain the progressive resistance to OTC associated with incremental exposure to the drug. The specific mutations consistently identified in isolates exposed to OTC were on AHA_ 2785 (associated with an outer membrane protein), AHA_2910 (involved in the efflux pump mechanism), and AHA_0308 (associated with the small ribosomal subunit protein S10). The pathways involved in the differential gene expression included efflux- pump mechanisms, outer membrane proteins, and ribosomal protein OTC target. Our findings support the notion that AMR can occur via genetic regulation of several intrinsic mechanisms within a bacterial population. This finding could have implications in aquaculture where bacteria such as A. hydrophila can be exposed to varying levels of antibiotics during in-feed treatments.

2025-10-01 | GSE290492 | GEO

Country can affect gut microbiome and antibiotic resistance genes.

Project description:Country can affect gut microbiome and antibiotic resistance genes.

| PRJEB41489 | ENA

Evaluation of Acquired Antibiotic Resistance in Klebsiella pneumoniae Exposed to Short-Term Simulated Microgravity and Background Antibiotic Exposure

Project description:Space flight missions last for a long time so bacterial infection during missions is considered a potential risk for astronauts. Studies of bacterial antibiotic resistance under spaceflight and simulated microgravity (SMG) have shown contrary results. To better understand the antibiotic stress resistance of K. pneumoniae in the microgravity environment, the original strain of K. pneumoniae (CGMCC 1.839), designated the KPO strain, was cultured under SMG conditions combined with background antibiotic exposure (SMGA) as the experimental group, while the control group was cultured in a NG environment without antibiotic exposure. After 20 cycles of incubation, the growth rate, antibiotic susceptibility, genomic , transcriptomic, and proteomic tests were conducted on the experimental and control groups designated the SMGA and NG strains, respectively.

2022-01-06 | PXD030787 |

Gut Microbiome and Common Antibiotic Resistance Genes

Project description:The Comparison of Gut Microbiome and Common Antibiotic Resistance Genes between Plateau Pika and Plateau Zokor

| PRJEB39107 | ENA

Metagenomics uncover antibiotic resistance genes in pig and chicken

Project description:Metagenomics uncover antibiotic resistance genes in pig and chicken Raw sequence reads

| PRJNA598414 | ENA