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Differential gene expression in two populations of the wood white butterfly (Leptidea sinapis) from Catalonia and Sweden reared on different host plants, Lotus dorycnium and Lotus corniculatus

ABSTRACT: Differential gene expression in two populations of the wood white butterfly (Leptidea sinapis) from Catalonia and Sweden reared on different host plants, Lotus dorycnium and Lotus corniculatus

PROVIDER: PRJEB40787 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4676810_1.fastq.gz	Fastqsanger.gz
	ERR4676810_2.fastq.gz	Fastqsanger.gz
	ERR4676820_1.fastq.gz	Fastqsanger.gz
	ERR4676820_2.fastq.gz	Fastqsanger.gz
	ERR4676830_1.fastq.gz	Fastqsanger.gz

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Differential gene expression in two populations of the wood white butterfly (Leptidea sinapis) from Catalonia and Sweden reared on different host plants, Lotus dorycnium and Lotus corniculatus

Project description:The gene expression study is part of a larger study with the objectives to investigate two populations of the wood white butterfly with different hostplant use, by assessing the female host plant preference, larval growth and developmental time, differential gene expression and microbiome diversity and composition. The aim of the gene expression analysis is to explore whether the two populations have different expression profiles in response to the host plants suggesting local adaptation. Larvae from four Swedish females and seven Catalonian females were reared on Lotus dorycnium or L. corniculatus in a split brood design experiment. Larvae were collected at two developmental timepoints, instar 3 and instar 5. In instar 5 one larvae of each sex were collected. The study included in total 66 individuals. RNA from the abdomen was extracted with RNeasy mini kit. Sample 67-72 are RNA from gut tissue extracted with Trizol. Total RNA-libraries was constructed using TruSeq RNA with poly-A selection for multiplex sequencing on a single Illumina NovaSeq6000 S1 lane with 150 bp paired-end reads at the National Genomics Infrastructure (NGI), Science for Life Laboratory (SciLife) in Stockholm. The average coverage was appr 248 X per gene and sample. The raw reads for each sample is included.

2020-11-01 | E-MTAB-9668 | biostudies-arrayexpress

Lotus corniculatus

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Leptidea sinapis RAD-seq pedigree

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Gene expression profiling across ontogenetic stages in wood white (Leptidea sinapis) reveals pathways linked to butterfly diapause regulation.

Project description:In temperate latitudes, many insects enter diapause (dormancy) during the cold season, a period during which developmental processes come to a standstill. The wood white (Leptidea sinapis) is a butterfly species distributed across western Eurasia that shows photoperiod-induced diapause with variation in critical day-length across populations at different latitudes. We assembled transcriptomes and estimated gene expression levels at different developmental stages in experimentally induced directly developing and diapausing cohorts of a single Swedish population of L. sinapis to investigate the regulatory mechanisms underpinning diapause initiation. Different day lengths resulted in expression changes of developmental genes and affected the rate of accumulation of signal molecules, suggesting that diapause induction might be controlled by increased activity of monoamine neurotransmitters in larvae reared under short-day light conditions. Expression differences between light treatment groups of two monoamine regulator genes (DDC and ST) were observed already in instar III larvae. Once developmental pathways were irreversibly set at instar V, a handful of genes related to dopamine production were differentially expressed leading to a significant decrease in expression of global metabolic genes and increase in expression of genes related to fatty acid synthesis and sequestration. This is in line with a time-dependent (hour-glass) model of diapause regulation where a gradual shift in the concentration of monoamine neurotransmitters and their metabolites during development of larvae under short-day conditions leads to increased storage of fat, decreased energy expenditures, and ultimately developmental stasis at the pupal stage.

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Philemon corniculatus strain:NA

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