Project description:Coumarin has been reported as a quorum sensing inhibitor for Pseudomonas aeruginosa. The goal of this transcriptomic analysis is to elucidate the effect of coumarin on gene expression of P. aeruginosa. Therefore, planktonic cells of P. aeruginosa were treated by coumarin for 1h and biofilms were formed in the presence of coumarin for 24h. Unreated controls (with dimethyl sulfoxide ) for both planktonic and biofilm samples were also included. Three biological replicates per treatment were performed with RNA sequencing.
Project description:With the general adoption of new approach methodologies, the omic-based technologies are of particular importance for chemical hazard characterization owning to the premise that any apical endpoint change indicative of impaired health must be underpinned by some alterations at the omic level. In this work we studied cellular response to coumarin by measuring transcriptomics experiments. The HepG2 cells were treated with 6 doses of coumarin for 24 h. 1 control group was set (0 uM of coumarin). Each group has 3 biological replicates.
Project description:With the general adoption of new approach methodologies, the omic-based technologies are of particular importance for chemical hazard characterization owning to the premise that any apical endpoint change indicative of impaired health must be underpinned by some alterations at the omic level. In this work we studied cellular response to coumarin by measuring transcriptomics experiments. The HepG2 cells were treated with 6 doses of coumarin for 6 h. 1 control group was set (0 uM of coumarin). Each group has 3 biological replicates.
Project description:This SuperSeries is composed of the following subset Series: GSE36341: mRNA degradation in Mycobacterium tuberculosis under aerobic conditions GSE36342: mRNA degradation in Mycobacterium smegmatis under aerobic conditions GSE36343: mRNA degradation in Mycobacterium tuberculosis during cold and hypoxic stress GSE36344: mRNA degradation in Mycobacterium tuberculosis with DosR ectopically induced Refer to individual Series
Project description:Transcriptomics analyses indicated that there were multiple genes differentially expressed between the root system and the canopy at different growth stages. These genes were associated with multiple pathways contributing to phenylalanine synthesis and metabolism. Moreover, the transcriptional activity of related enzyme-encoding genes did not differ significantly at each step of the pathway from the synthesis of umbelliferone to the formation of various coumarin compounds. Metabolomics analyses of the root and canopy indicated 491 differentially accumulated metabolites were involved in 82 metabolic pathways during the vegetative growth stage, while 572 differentially accumulated metabolites were associated with 76 metabolic pathways during the reproductive growth stage. Additionally, 307 differentially accumulated metabolites in both growth stages were involved in 14 metabolic pathways, many of which were related to coumarin biosynthesis.
Project description:Four stable and robust TCE-dechlorinating microbial communities were enriched from TCE-contaminated groundwater under four different conditions exploring two parameters, high and low methanogenic activity (Meth and NoMeth), with and without vitamin B12 supplement (MethB12 and NoMethB12, Meth and NoMeth, respectively). Identical amounts of lactate (2.7 mmol) and TCE (20 μl) were supplied as electron donor and electron acceptor. All four cultures were capable of reductively dechlorinating TCE to VC and ethene. Genomic DNA of the four enrichments was applied on a quad-Dhc-genome microarray in order to characterize the gene content of Dehalococcoides species present in the four enrichments
Project description:To investigate how Methanothermobacter marburgensis adapts to nickel limitation, we performed transcriptomic profiling under 50 nM and 5000 nM nickel conditions. RNA-seq revealed differential expression of hydrogenase-related genes, suggesting a shift in methanogenic electron flow under nickel limitation, with increased reliance on [Fe]-hydrogenase and associated pathways.
2026-01-06 | GSE311913 | GEO
Project description:Methanogenic n-alkanes degradation microbial community