Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of CTR and dod mutant Drosophila heads

ABSTRACT: RNA-seq of CTR and dod mutant Drosophila heads

PROVIDER: PRJEB41707 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR4913720_1.fastq.gz	Fastqsanger.gz
	ERR4913720_2.fastq.gz	Fastqsanger.gz
	ERR4915900_1.fastq.gz	Fastqsanger.gz
	ERR4915900_2.fastq.gz	Fastqsanger.gz
	ERR4913402_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Oxidative stess in Drosophila heads

Project description:Profiled the effect of oxidative stress on gene expression in the heads of adult Drosophila. The flies were fed sucrose with 15mM paraquat (experimental condition) or regular sucrose (control condition) for 30 hours, before harvesting at adult d3. Keywords = oxidative stress Keywords = paraquat Keywords: dose response

2004-01-18 | GSE827 | GEO

RNA-Seq of Heliconius melpomene heads

Project description:We made RNA-Seq libraries of Heliconius melpomene heads, antennae, legs and mouth parts to identify genes upregulated in heads. We explored the expression patterns of a gene family that functions in visual pigment chromophore transport in Drosophila to identify a non-orthologous gene that has a similar function.

2018-02-28 | E-MTAB-6342 | biostudies-arrayexpress

Gene expression in Drosophila female heads during diapause.

Project description:Young Drosophila females respond to low temperature and short photoperiod by developmental arrest of the ovaries. This form of reproductive diapause is a winter adaptation. Here we used Affymetrix microarrays to study global expression in female heads associated with diapause.

2014-07-18 | GSE28370 | GEO

RNAseq of Zcchc8 mutant mouse embryonic heads

Project description:RNAseq of Zcchc8 mutant mouse embryonic heads

| PRJNA521035 | ENA

Gel-free mass spectrometry analysis of Drosophila melanogaster heads

Project description:Here we describe a bottom-up approach to analyse an enriched membrane fraction from Drosophila melanogaster heads using multidimensional liquid chromatography (LC) coupled with tandem-mass spectrometry (MS/MS) that relies on a complete solubilisation and digestion of proteins. An enriched membrane fraction was prepared using equilibrium density centrifugation on a discontinual sucrose gradient, followed by solubilisation using FASP method, tryptic and consequential chymotryptic digestion of proteins. Peptides were separated by reversed-phase (RP) LC at high pH in the first dimension and acidic RP-LC coupled directly to Orbitrap Velos Pro mass spectrometer.

2015-07-27 | PXD001712 | Pride

Transcription profiling of Drosophila heads after aging and oxidative stress

Project description:This SuperSeries is composed of the following subset Series:; GSE826: Aging in heads from adult Drosophila males, w118 strain; GSE827: Oxidative stess in Drosophila heads Experiment Overall Design: Refer to individual Series

2008-06-14 | E-GEOD-6430 | biostudies-arrayexpress

ChIP-Seq of V5-Clk in wildtype and tim promoter mutant Drosophila heads around the clock

Project description:To address the contribution of transcriptional regulation to Drosophila clock gene expression and to behavior, we generated a series of CRISPR-mediated deletions within two regions of the circadian gene timeless (tim), an intronic E box region and an upstream E box region that are both recognized by the key transcription factor Clock (Clk) and its heterodimeric partner Cycle. The upstream deletions but not an intronic deletion dramatically impact tim expression in fly heads; the biggest upstream deletion reduces peak RNA levels and tim RNA cycling amplitude to about 15% of normal, and there are similar effects on tim protein (TIM). The cycling amplitude of other clock genes is also strongly reduced, in these cases due to increases in trough levels. To examine the effect of promoter E-box deletions on Clk binding, we performed Clk-ChIP around the clock in wild-type and 126 mutant flies.

2024-02-28 | GSE259247 | GEO

RNA-Seq of head tissue from Drosophila melanogaster Wild Type and Adar5G1dAdar-/- mutant

Project description:Purpose: Validation of Drosophila A-to-I editing sites Methods: We collected heads of 5 day old male dAdar-/- mutant (y, Adar5G1, w)26 and wild type (w1118) flies. Poly(A)+ RNA was used to prepare RNA-seq libraries which were subsequently sequenced single-end by an Illumina GAII Results:We builded a framework to identify RNA editing events using RNA-seq data alone in Drosophila. To validate whether the identified A-to-G sites were bona fide A-to-I editing events, we performed RNA-seq for the D.melanogaster wild-type strain (w1118) and for the Adar5G1 null mutant that eliminates RNA editing. We found that our method achieved high accuracy; 98.2% of all A-to-G sites showed only adenosine in the Adar5G1 sample Conclusions: We anticipate that our method will be very effective in the future to identify RNA editing events in different species. mRNA profiles of heads of 5 day old male dAdar-/- mutant (y, Adar5G1, w)26 and wild type (w1118) flies

2012-12-20 | E-GEOD-42815 | biostudies-arrayexpress

Transcription profiling of Drosophila heads

2008-06-16 | E-GEOD-827 | biostudies-arrayexpress

ChIP-Seq of V5-Clk in wildtype and tim promoter mutant Drosophila heads around the clock

Project description:ChIP-Seq of V5-Clk in wildtype and tim promoter mutant Drosophila heads around the clock

| PRJNA1080521 | ENA