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ScRNAseq of BRAF-mutant Colo205 cells control or treated for 24h or 72h with vemurafenib

ABSTRACT: scRNAseq of BRAF-mutant Colo205 cells control or treated for 24h or 72h with vemurafenib

PROVIDER: PRJEB41852 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4972711_1.fastq.gz	Fastqsanger.gz
	ERR4972711_2.fastq.gz	Fastqsanger.gz
	ERR4972712_1.fastq.gz	Fastqsanger.gz
	ERR4972712_2.fastq.gz	Fastqsanger.gz
	ERR4972713_1.fastq.gz	Fastqsanger.gz

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scRNAseq of BRAF-mutant Colo205 cells control or treated for 24h or 72h with vemurafenib

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Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of RNAs in BRAFV600E+ thyroid cancer cell lines 8505C and WRO. We generated genome-wide transcriptome maps of 8505C and WRO cells after vemurafenib, BRAF inhibitor, treatment. In addition, we generated genome-wide transcriptome maps of clonally selected vemurafenib-resistant 8505C and WRO cells with or without vemurafenib treatment. Negative control treatment was 0.1% of dimethy solfoxide (DMSO), the diluent used to resuspend vemurafenib. The treatment regine of vemurafenib was 2 µM concentration for 24 hours. We find that both inducible response to BRAFi and acquired BRAFi resistance in thyroid cancer cells showed significant activation of the JAK/STAT pathway. Our study demonstrates that the JAK/STAT-signaling pathway is activated as thyroid cancer cells develop resistance to BRAFi and that this pathway is a potential target for anticancer activity and to overcome drug resistance that commonly develops to treatment with BRAFi in thyroid cancer and potentially in other malignancies.

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Expression data from BRAFV600E A375 melanoma cells treated with vehicle or vemurafenib

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