Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Germinal Center B-Cell gene expression comparison between Wiskott–Aldrich syndrome protein KO mice and WT mice

ABSTRACT: Germinal Center B-Cell gene expression comparison between Wiskott–Aldrich syndrome protein KO mice and WT mice

PROVIDER: PRJEB42708 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR5195750.fastq.gz	Fastqsanger.gz
	ERR5195751.fastq.gz	Fastqsanger.gz
	ERR5195752.fastq.gz	Fastqsanger.gz
	ERR5195753.fastq.gz	Fastqsanger.gz
	ERR5195754.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Germinal Center B-Cell gene expression comparison between Wiskott-Aldrich syndrome protein KO mice and WT mice

Project description:This experiment intended to define differential gene expression between germinal center B cells expressing or not the Wiskott-Aldrich syndrome protein in mice. Sequencing was obtained on an Illumina HiSeq2500 system from Dark Zone GCB (DAPI-CD19+ GL7+IgD-CXCR4highCD86low) purified from CTL and GCBcWKO mice (n=4).

2021-06-01 | E-MTAB-10056 | biostudies-arrayexpress

Proteome profiling of mouse germinal center B cells

Project description:Productive B cell responses are critical to protect a host from infection. The spleen and lymph nodes are populated by resting follicular B cells, which can enter germinal centers upon antigen encounter. Once in the germinal center, B cells migrate between the dark and light zones, where they undergo somatic hypermutation and selection, respectively. While germinal center B cells have been studied, an intense molecular understanding of these cells/subsets (and the differences between them) is lacking.

2020-04-06 | PXD015524 | Pride

A novel WASP splice-site mutation in a patient with Wiskott-Aldrich syndrome

Project description:gene panel of patient with Wiskott-Aldrich syndrome

| PRJEB75037 | ENA

Single-cell gene expression of germinal center B cells from CXCL12-conditionally deficient and –sufficient mice

Project description:The chemokines CXCL13 and CXCL12 are reported to be important for the germinal center reaction. Since CXCL12-deficient mice are embryonically lethal, here we took advantage of the Cxcl13-Cre/TdTomato mouse models to genetically ablate CXCL12 from B cell-interacting reticular cells and examine the molecular consequence on germinal center B cells. Spatial segregation of follicular dendritic cells, germinal center B cells and follicular helper T cells is impaired in Cxcl13-Cre/TdTomato Cxcl12fl/fl mice. Single cell transcriptomic analysis revealed that all germinal center B cell subsets (corresponding to distinct stages of the germinal center response) are present in draining lymph nodes of immunized CXCL12-conditionally deficient mice. While most transcriptional regulators of the germinal center response are unperturbed by the genetic perturbation of CXCL12, Bach2 levels were elevated in germinal center B cells from lymph nodes of Cxcl12fl/fl mice. Moreover, single cell B cell receptor sequencing revealed that germinal center B cells in Cxcl13-Cre/TdTomato Cxcl12fl/fl mice harbour a lower mutational burden when compared to germinal center B cells isolated from immunized control mice. Gene expression profiles were validated by flow cytometry and suggest that the provision of CXCL12 by reticular cells governs efficient germinal center responses.

2020-05-12 | E-MTAB-8454 | biostudies-arrayexpress

The influence of TFEB on gene expression in primary murine germinal center and non-germinal center B cells

Project description:To investigate TFEB-dependent mRNA expression in murine B cells, FACS-sorted germinal center and non-germinal center B lymphocytes of B-cell-specific conditional TFEB KO mice and control littermates were subjected to RNA sequencing analysis

2024-06-25 | GSE237799 | GEO

SFR KO mice germinal center B cell RNA sequencing

Project description:WT-AID-GFP+ and SFR KO-AID-GFP+ mice were immunized with NP(18)OVA+ alum, on Day 9, germinal center B cells (AID-GFP+) were sorted for total RNA

2020-11-16 | GSE147990 | GEO

Transcriptome analysis of germinal center B cells of WT and SAP-deficient mice

Project description:Analysis of germinal center B cells derived from WT and SAP-deficient mice revealed that SAP-deficient mice have reduced Myc signature

2019-12-28 | GSE142640 | GEO

ChIPseq analyses in germinal center B cells

Project description:Germinal center B cells were isolated from human tonsil tissue and crosslinked. ChIP was performed on two distinct pools of germinal center cells, each obtained from 3-5 donors. The experiment includes two biological replicates (germinal center cell pools from different donors). ChIP was performed on both pools and subject to library preparation and sequencing. Input DNA was sequenced for both pools.

2015-12-04 | E-GEOD-68349 | biostudies-arrayexpress

SFR KO mice germinal center B cell RNA sequencing

Project description:SFR KO mice germinal center B cell RNA sequencing

| PRJNA622768 | ENA

ChIPseq analyses in germinal center B cells

2015-12-04 | GSE68349 | GEO