Project description:RNAseq was performed to understand the role of SMRT in CD8α+ DCs. MutuDC cell line was transduced with empty and smrt shrna lentiviral particles and cells were prepared in unstimulated or 6hr CpG stimulation condition.
Project description:RNAseq were performed to understand the role of SMRT in CD8α+ DCs. MutuDC line were transduced with empty vector and SMRT shRNA using lentivirus and cells were prepared in unstimulated or 6hr CpG stimulation condition.
Project description:RNA-seq was performed to understand the role of NCoR1 in CD8α+ DCs. MutuDC cell line was transduced with empty and Ncor1 shrna lentiviral particles and cells were prepared in unstimulated, 2hr and 6hr (IFNg, pIC and CpG+pIC+IFNg) stimulation condition.
Project description:H3K27ac ChIP-seq was performed to identify active enhancers in different TLR activated control CD8α+ DCs as well as to understand the role of NCoR1 in regulating TLR specific enhacner activity using H3K27ac ChIP-seq performed in NCoR1 KD DCs. MutuDC1940 cell line were treated with TLR3 ligand poly I:C, TLR9 ligand CpG-B and combined stimulation of TLR9 , TLR3, and IFNg for 2hr and 6hr. Recombinant Anti-Histone H3 (acetyl K27) antibody was used to pull down chromatin regions having active enhacner mark in each unstimulated and stimulated condition.