Transcriptional response of Arabidopsis thaliana to exogenous application of N-hydroxypipecolic acid (NHP).
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ABSTRACT:
PROVIDER: PRJEB43717 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR5489950.fastq.gz | Fastqsanger.gz | |||
| ERR5489960.fastq.gz | Fastqsanger.gz | |||
| ERR5489951.fastq.gz | Fastqsanger.gz | |||
| ERR5489961.fastq.gz | Fastqsanger.gz | |||
| ERR5489952.fastq.gz | Fastqsanger.gz |
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Project description:Previous studies from our laboratory have shown that the resistance increase observed after biological systemic acquired resistance (SAR) induction in plants can be mimicked by exogenous plant treatment with N-hydroxypipecolic acid (NHP, Hartmann et al., 2018, Schnake et al., 2020). Moreover, exogenous application of the NHP biosynthetic precursor pipecolic acid (Pip) induced a transcriptional response that was overlapping with the SAR transcriptional response and fully depended on the NHP synthase FMO1 (Hartmann et al., 2018; E-MTAB-6243). In order to investigate whether elevations of NHP lead to a SAR-like transcriptional reprogramming, we supplied individual Arabidopsis wildtype Col-0 plants, as well as sid2-1 and npr1-3 mutant plants, with doses of 10 µmol NHP and determined the transcriptional response in leaves 24 hours later on the whole genome level by RNA-sequencing analyses in relation to control-treated (H2O) plants. Col-0 plants were additionally treated with doses of 10 µmol Pip in order to directly compare the transcriptional responses of the foliage between Pip and NHP. Arabidopsis thaliana plants were grown individually in pots containing a mixture of soil, vermiculite and sand (8:1:1) in a controlled cultivation chamber with a 10-h day (9 AM to 7 PM; photon flux density 100 mol m-2 s-1) / 14-h night cycle and a relative humidity of 70 %. Day and night temperatures were set to 21°C and 18°C, respectively. Experiments were performed with 5-week-old, naive plants exhibiting a uniform appearance. Treatments with NHP and Pip were essentially performed as detailed in Hartmann et al. (2018, Cell 173, 456–469). In brief, 10 ml of a 1 mM aqueous solution of NHP or Pip (equates to a dose of 10 µmol) were pipetted onto the soil of individually cultivated plants. 10 ml of water applied in the way served as control treatments. In total, three biologically independent, replicate experiments were performed. In each experiment, 18 full-grown leaves from 6 different plants were pooled 24 hours after the respective treatments for one biological replicate. In this way, 3 biologically independent, replicate samples per treatment and plant genotype were obtained.
2021-05-03 | E-MTAB-10230 | biostudies-arrayexpress
Project description:Transcriptional response of Arabidopsis thaliana to exogenous application of pipecolic acid
| PRJEB23627 | ENA
Project description:N-hydroxy-pipecolic acid (NHP) is a mobile metabolite essential for inducing and amplifying systemic acquired resistance (SAR) following pathogen attack. In Arabidopsis thaliana (Arabidopsis), exogenous NHP is sufficient to activate SAR-related immune responses, including salicylic acid (SA) accumulation and changes in global transcriptional profile. Previous studies have tracked these changes 1-2 days after an initial NHP treatment, resulting in little knowledge of the very early phases of NHP signaling and transcriptional responses leading to immunity. Here we show NHP elicits transcriptional changes within minutes of treatment. We report distinct waves of expression over the course of minutes to hours defined by transient induction of jasmonic acid/wound-related responses, a primary induction of WRKY transcription factor expression, and subsequent induction of WRKY-regulated defense genes. The upregulation of WRKYs and the majority of defense-related genes occurred in the sid2-2 mutant, which is unable to accumulate SA upon NHP treatment, suggesting NHP is sufficient to drive the early phase of transcriptional changes in a low SA environment. We also show that WRKY70 is required for the expression of a set of genes defining the secondary transcriptional changes, as well as NHP enhancement of ROS production and SAR.
2024-08-16 | GSE263276 | GEO
Project description:We have previously shown that the resistance increase observed after biological systemic acquired resistance (SAR) induction in plants can be mimicked by exogenous plant treatment with (pipecolic acid) Pip (Návarová et al., 2012; Bernsdorff et al., 2016). In order to investigate whether elevations of Pip are sufficient for a SAR-like transcriptional reprogramming in leaf tissue, we supplied individual Arabidopsis Col-0 and fmo1 mutant plants with doses of 10 µmol Pip and determined the transcriptional response in leaves one day later on the whole genome level by RNA-sequencing analyses in relation to untreated plants. Arabidopsis thaliana plants were grown individually in pots containing a mixture of soil, vermiculite and sand (8:1:1) in a controlled cultivation chamber with a 10-h day (9 AM to 7 PM; photon flux density 100 mol m-2 s-1) / 14-h night cycle and a relative humidity of 70 %. Day and night temperatures were set to 21°C and 18°C, respectively. Experiments were performed with 5- to 6-week-old, naive plants exhibiting a uniform appearance.Treatments with Pip were essentially performed as detailed in Návarová et al. (2012).10 ml of a 1 mM aqueous solution of Pip (equates to 10 µmol) were pipetted onto the soil of individually cultivated plants. 10 ml of water were applied in the control treatments. Three biologically independent, replicate experiments were performed. In each experiment, at least 4 full-grown leaves from 6 different plants were pooled 24 hours after the treatment with Pip or water for one biological replicate. In this way, 3 biologically independent, replicate samples per treatment and plant genotype were obtained.
2018-03-23 | E-MTAB-6243 | biostudies-arrayexpress
Project description:N-hydroxy-pipecolic acid (NHP) primes Aarabidopsis thaliana to confer faster and stronger activation of immune responses, but how NHP interacts with other immune-inducing phytohormones is unknown. Here we investigated the crosstalk between priming phytohormone NHP and immune inducing phytohormone salicylic acid (SA). We show that pretreatment with NHP modulates the SA-inducible transcriptome, wherein some SA-expressed genes show enhanced SA-sensitivity and some show reduced SA-sensitivity. 12 day old wild type (col-0) seedlings grown on MS media were treated with 1mM NHP or water overnight, then 0.5mM SA or water for 6 hours by immersion in 10ml of the respective chemicals. ~50 seedlings were pooled into one biological repeat, with three biological repeats total for this experiment. Seedlings were patted dry with tissue paper and frozen in liquid nitrogen for RNA extraction.
2024-12-25 | E-MTAB-14631 | biostudies-arrayexpress
Project description:N-hydroxy-pipecolic acid (NHP) primes Aarabidopsis thaliana to confer faster and stronger activation of immune responses, in part by priming salicylic acid (SA) signalling. Here we investigated the potential regulators of NHP-SA crosstalk in priming. We show that in wrky38/wrky62 double mutants, SA signalling is minimally affected but the NHP priming effect is greatly attenuated, with most wild type primed genes (identified in E-MTAB-14631) no longer NHP responsive. 12 day old wrky38 (SAIL_749_B02)/wrky62 (SM_3_38820) double mutant seedlings grown on MS media were treated with 1mM NHP or water overnight, then 0.5mM SA or water for 6 hours by immersion in 10ml of the respective chemicals. ~50 seedlings were pooled into one biological repeat, with three biological repeats total for this experiment. Seedlings were patted dry with tissue paper and frozen in liquid nitrogen for RNA extraction.
2025-03-01 | E-MTAB-14847 | biostudies-arrayexpress
Project description:This study evaluates the effects of exogenous auxin on the Arabidopsis thaliana root proteome at 8, 12, and 24 hours post-treatment.
2017-07-18 | PXD001400 | Pride
Project description:siRNA generation in reponsse to exogenous dsRNA application in Paramecium
| PRJEB86902 | ENA
Project description:Transcriptional response of Arabidopsis thaliana to exogenous application of pipecolic acid
2018-06-04 | E-MTAB-6243 | ExpressionAtlas
Project description:The goal of the microarray was to investigate the transcriptome changes induced by exogenous NAD+ in the wild-type Col-0 plants. Results showed that exogenous NAD+-induced dramatic transcriptional changes in Arabidopsis. Particularly, a large group of salicylic acid pathway genes including NPR1 and its traget genes were induced by NAD+, whereas the jasmonic acid/ethylene pathway defense marker gene PDF1.2 was inhibited by NAD+ treatment. In addition, a group of the pathogen-associated molecular pattern pathway genes were also induced by exogenous NAD+. These results indicate that exogenous NAD+ induces defense pathways against (hemi)biotrophic pathogens but suppresses defense against necrotrophs.
2016-01-07 | GSE76568 | GEO